flyrnai.blogspot.com
FlyRNAi: Glia-specific RNAi screen explores circadian behavior
http://flyrnai.blogspot.com/2015/07/glia-specific-rnai-screen-explores.html
Monday, July 27, 2015. Glia-specific RNAi screen explores circadian behavior. Ng FS, Jackson FR. The ROP vesicle release factor is required in adult Drosophila glia for normal circadian behavior. Front Cell Neurosci. 2015 Jul 3;9:256. PMID: 26190976. Labels: in vivo assay development. Subscribe to: Post Comments (Atom). Drosophila RNAi Screening Center (DRSC) Database and Website. Bloomington Drosophila Stock Center. Drosophila Genome Resource Center. Drosophila Models of Human Disease. In vivo RNAi cont...
flyrnai.blogspot.com
FlyRNAi: October 2014
http://flyrnai.blogspot.com/2014_10_01_archive.html
Thursday, October 30, 2014. Full-genome screen related to functional insulators. Another full-genome DRSC screen published- congrats! Fig 1 from the Bohla et al. paper. Bohla D, Herold M, Panzer I, Buxa MK, Ali T, Demmers J, Krüger M, Scharfe M, Jarek M, Bartkuhn M, Renkawitz R. A Functional Insulator Screen Identifies NURF and dREAM Components to Be Required for Enhancer-Blocking. PLoS One. 2014 Sep 23;9(9):e107765. PubMed PMID: 25247414. PubMed Central PMCID: PMC4172637. Thursday, October 16, 2014.
flyrnai.blogspot.com
FlyRNAi: DRSC publishes RBP library resource including 'baseline data' available lots of ways--including interactive!
http://flyrnai.blogspot.com/2015/07/drsc-publishes-rbp-library-resource.html
Wednesday, July 22, 2015. DRSC publishes RBP library resource including 'baseline data' available lots of ways- including interactive! The DRSC is pleased to announce our new cell-based RNAi library targeting RNA Binding Proteins and corresponding 'baseline' data set. These data should be a helpful reference for anyone analyzing data from screens of the same library with more sophisticated assays. Big thanks to all the folks who worked hard to make this possible! Early online at G3 July 2015. The goals o...
flyrnai.blogspot.com
FlyRNAi: December 2014
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Monday, December 15, 2014. Thinking ahead- What could custom CRISPR engineered cell lines do for your research? Open call to the Drosophila. Research community from the DRSC. Since it was founded in 2003 by Prof. N. Perrimon, the Drosophila. RNAi Screening Center (DRSC) has served as a technology transfer center, helping the Drosophila. Community-at-large gain access to leading-edge technologies such as genome-wide RNAi. In short, we need your help! If custom engineered cells would help your research.
flyrnai.blogspot.com
FlyRNAi: Drosophila in vivo RNAi screen explores ovarian follicle cell differentiation
http://flyrnai.blogspot.com/2015/07/drosophila-in-vivo-rnai-screen-explores.html
Monday, July 27, 2015. Drosophila in vivo RNAi screen explores ovarian follicle cell differentiation. Jia D, Soylemez M, Calvin G, Bornmann R, Bryant J, Hanna C, Huang YC, Deng WM. A large-scale in vivo RNAi screen to identify genes involved in Notch-mediated follicle cell differentiation and cell cycle switches. Sci Rep. 2015 Jul 24;5:12328. PMID: 26205122. Labels: in vivo assay development. Subscribe to: Post Comments (Atom). Drosophila RNAi Screening Center (DRSC) Database and Website. New report on T...
flyrnai.blogspot.com
FlyRNAi: March 2015
http://flyrnai.blogspot.com/2015_03_01_archive.html
Monday, March 30, 2015. NIH NIGMS announces new strategic plan. Many readers will be interested to learn that NIGMS at NIH has released a new strategic plan. An announcement, including a link to a PDF copy of the plan, is at the NIGMS Feedback Loop blog. Report of 'systems-level interrogation' includes DRSC RNAi screen data, phosphoproteomics and in vivo analyses. PLoS Genet. 2015 Mar 6;11(3):e1005056. PMID: 25749252. Labels: cell line selection. Friday, March 27, 2015. Wednesday, March 25, 2015. We intr...
flyrnai.blogspot.com
FlyRNAi: April 2015
http://flyrnai.blogspot.com/2015_04_01_archive.html
Friday, April 10, 2015. Transcription factor validated in follow-up to genome-wide RNAi screen related to viral-host cell interactions. Panda D, Gold B, Tartell MA, Rausch K, Casas-Tinto S, Cherry S. The Transcription Factor FoxK Participates with Nup98 To Regulate Antiviral Gene Expression. MBio 2015 Apr 7;6(2). PMID: 25852164. In vivo screen of kinases and phosphatases identifies Wnt signal regulators. Development. 2015 Apr 15;142(8):1502-15. PMID: 25852200. Labels: in vivo assay development. Modest le...
flyrnai.blogspot.com
FlyRNAi: CRISPR activation in vivo in Drosophila
http://flyrnai.blogspot.com/2015/08/crispr-activation-in-vivo-in-drosophila.html
Thursday, August 6, 2015. CRISPR activation in vivo in Drosophila. Things are looking up: #CRISPR. Activation in vivo in #Drosophila. Early online in #Genetics. Mdash; Stephanie Mohr (@smohrfly) August 6, 2015. Subscribe to: Post Comments (Atom). Drosophila RNAi Screening Center (DRSC) Database and Website. Bloomington Drosophila Stock Center. Drosophila Genome Resource Center. Drosophila Models of Human Disease. Drosophila Species Stock Center. FlyPrimerBank (qPCR primer info resource).
flyrnai.blogspot.com
FlyRNAi: June 2015
http://flyrnai.blogspot.com/2015_06_01_archive.html
Wednesday, June 24, 2015. Search with "Drosophila" now returns 90,000 results in PubMed! Now returns 90,000 results at PubMed. Let the count down to 100,000 begin! 8212; Stephanie Mohr (@smohrfly) June 24, 2015. Genetic toolkit" for intronic MiMICs. Nagarkar-Jaiswal S, DeLuca SZ, Lee PT, Lin WW, Pan H, Zuo Z, Lv J, Spradling AC, Bellen HJ. A genetic toolkit for tagging intronic MiMIC containing genes. Elife 2015 Jun 23;4. PMID: 26102525. PLoS One. 2015 May 29;10(5):e0128101. PMID: 26023770. Fulga TA, McN...
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FlyRNAi: November 2014
http://flyrnai.blogspot.com/2014_11_01_archive.html
Wednesday, November 19, 2014. Review and detailed protocols- CRISPR-Cas9 in flies and fly cells. Housden BE, Lin S, Perrimon N. Cas9-based genome editing in Drosophila. Methods Enzymol. 2014;546:415-39. PMID: 25398351. We first discuss some general design principles for genome engineering experiments in Drosophila and then present detailed protocols for the production of CRISPR reagents and screening strategies to detect successful genome modification events in both tissue culture cells and animals.".