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Blue Collar Bioinformatics

(by Brad Chapman)

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Blue Collar Bioinformatics | bcb.io Reviews
<META>
DESCRIPTION
(by Brad Chapman)
<META>
KEYWORDS
1 blue collar bioinformatics
2 overview
3 finally bina
4 miika ahdesmaki
5 and justin johnson
6 at astrazeneca
7 luca beltrame
8 lorena pantano
9 variant caller validation
10 labs
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blue collar bioinformatics,overview,finally bina,miika ahdesmaki,and justin johnson,at astrazeneca,luca beltrame,lorena pantano,variant caller validation,labs,and structural variants,improvements in filtering,tumor only prioritization,clinvar,and exac
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Blue Collar Bioinformatics | bcb.io Reviews

https://bcb.io

(by Brad Chapman)

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1

Updated comparison of variant detection methods: Ensemble, FreeBayes and minimal BAM preparation pipelines – Blue Collar Bioinformatics

http://bcb.io/2013/10/21/updated-comparison-of-variant-detection-methods-ensemble-freebayes-and-minimal-bam-preparation-pipelines

Community built tools for biological data analysis. Updated comparison of variant detection methods: Ensemble, FreeBayes and minimal BAM preparation pipelines. October 21, 2013. I previously discussed our approach for evaluating variant detection methods. Using a highly confident set of reference calls. Provided by NIST's Genome in a Bottle consortium. For the NA12878 human HapMap genome. In this post, I'll update those conclusions based on recent improvements in GATK and FreeBayes. Post-alignment BAM pr...

2

Validating multiple cancer variant callers and prioritization in tumor-only samples – Blue Collar Bioinformatics

http://bcb.io/2015/03/05/cancerval

Community built tools for biological data analysis. Validating multiple cancer variant callers and prioritization in tumor-only samples. March 05, 2015. The post discusses work validating multiple cancer variant callers in bcbio-nextgen. Using a synthetic reference call set from the ICGC-TCGA DREAM challenge. We've previously validated germline variant calling methods. Publicly available whole genome truth sets like the NA12878 Genome in a Bottle reference materials. Provides a set of synthetic datasets.

3

Validated whole genome structural variation detection using multiple callers – Blue Collar Bioinformatics

http://bcb.io/2014/08/12/validated-whole-genome-structural-variation-detection-using-multiple-callers

Community built tools for biological data analysis. Validated whole genome structural variation detection using multiple callers. August 12, 2014. Structural variant detection goals. This post describes community based work integrating structural variant calling and validation into bcbio-nextgen. I've previously written about approaches for validating single nucleotide changes (SNPs) and small insertions/deletions (Indels). X2013; a probabilistic structural variant caller. Developed by Tobias Rausch.

4

Validating generalized incremental joint variant calling with GATK HaplotypeCaller, FreeBayes, Platypus and samtools – Blue Collar Bioinformatics

http://bcb.io/2014/10/07/joint-calling

Community built tools for biological data analysis. Validating generalized incremental joint variant calling with GATK HaplotypeCaller, FreeBayes, Platypus and samtools. October 07, 2014. Incremental joint variant calling. There are two issues with trying to provide complete combined call sets. First, it is computationally expensive to call a large number of samples simultaneously. Second, adding any new samples to a callset requires repeating this expensive computation. This N 1 problem. Output for each...

5

Benchmarking variation and RNA-seq analyses on Amazon Web Services with Docker – Blue Collar Bioinformatics

http://bcb.io/2014/12/19/awsbench

Community built tools for biological data analysis. Benchmarking variation and RNA-seq analyses on Amazon Web Services with Docker. December 19, 2014. We developed a freely available, easy to run implementation of bcbio-nextgen. On Amazon Web Services (AWS). The entire installation bootstraps from standard Linux AMIs, enabling adjustment of the tools, genome data and installation without needing to prepare custom AMIs. The implementation uses Elasticluster. Isolates code and tools inside a Docker. With 1...

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Linux · kde. Danbooru Client 0.5.0 released. A new version of Danbooru Client is now available! Tip: opening and closing ports needed by a systemd service. Recently I’ve been testing out murmur, http:/ wiki.mumble.info/wiki/Main Page. Server component, on my CentOS 7 server. Murmur requires specific ports being open to operate, and when using it I would open them manually, and close them after the session had been completed. General · linux · kde. The Big Forum Cleaning. Danbooru Client 0.4.0. Content li...

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This project has been discontinued. You may want to look at Pandas. I have a problem. Danbooru Client 0.6.0 released. Of gases, Qt, and Wayland. Where are my noble gases? I need MORE noble gases! The ST.E.A.L. Saga. Dennogumi.org is a collection of blog posts on science, anime, and Free Software. More. Melissa Adkins - graphic designer. Content licensed under a CC BY-NC-SA 4.0.

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Validating multiple cancer variant callers and prioritization in tumor-only samples. Leave a comment ». The post discusses work validating multiple cancer variant callers in bcbio-nextgen. Using a synthetic reference call set from the ICGC-TCGA DREAM challenge. We’ve previously validated germline variant calling methods. Publicly available whole genome truth sets like the NA12878 Genome in a Bottle reference materials. Provides a set of synthetic datasets. That they used to compare multiple callers.

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