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Bioisis: welcome - indexThe resource for the investigation of macromolecular structures by SAXS
http://www.bioisis.net/
The resource for the investigation of macromolecular structures by SAXS
http://www.bioisis.net/
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View this contact
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Bioisis: welcome - index | bioisis.net Reviews
https://bioisis.net
The resource for the investigation of macromolecular structures by SAXS
Bioisis: experiments - details
http://www.bioisis.net/experiments/111
RPA DNA-Binding Core with 20mer". Your browser doesn't support canvas. Experimental Details for BID: DBC20Y. ALS BL 12.3.1. Brosey, CA , Yan, C , Tsutakawa, SE , Heller, WT , Rambo, RP , Tainer, JA , Ivanov, I , Chazin, WJ. Data was collected as a concentration series (1X, 2X, 4X) from 0.29 mL gel filtration fractions collected from a Superdex 200 HR 10/30 gel filtration column. The reported profile is from the 4X sample with a concentration of 10-11 mg/mL. Back to top →. Rarr; 114 Å. Back to top →.
Bioisis: news - index
http://www.bioisis.net/news
Scatter has been updated to release 3.0. The program was completely re-written and utilizes several new structures for handling datasets. PDB. RECONSTRUCTION OF SAXS PROFILES FROM PROTEIN STRUCTURES. Excellent review on SAXS. This should be required reading material for anyone wanting to learn SAXS. Comput Struct Biotechnol J. 2013; 8: e201308006. DENFERT: BEAD MODELING WITH HYDRATION. J Appl. Cryst. (2013). 46, 1884-1888. SCATTER 2.2B RELEASED. Scatter 2.2b has been released and features several upd...
Bioisis: tutorials - index
http://www.bioisis.net/tutorial
The information contained within this tutorial is introductory material aimed at new and general users of biological SAXS. Our goal is to remove the mystery of SAXS and provide some concrete examples for quantitive analysis of SAXS data quality. The menu items (on the left) are arranged as steps that one would typically take during the analysis of data.
Bioisis: tutorials - show
http://www.bioisis.net/tutorial/8
DATA DEPOSITING AND SUBMISSION. A deposit requires the experimental SAXS curve and associated P(r)-distribution to be uploaded as two separate column delimited text files. Any additional models, such as ab initio. Or homology models must be uploaded as separate PDB files. The following will be required:. 3 column text file, column 1: q, column 2: Intensity, column 3: error ). 2 column text file, column 1: distance, column 2: P(r) ). I(zero), Porod Volume, Real space R.
Bioisis: welcome - index
http://www.bioisis.net/welcome
Welcome to BIOISIS . the resource for macromolecular SAXS. Splicing factor complex of phosphorylated (P)SF1 residues 1-255 and U2AF65 residues 148-375. Human SF1 ULM-KH-QUA2, which is phosphorylated on Ser80Ser82, bound to U2AF65 RRM1-RRM2-UHM in a splicing factor complex. SAS Tool Box (SASTBX). TAPBPR (TAP Binding Protein, Related). SAXS analysis of Hsp104 wild-type and . RPA DNA-Binding Core with 20mer. RECONSTRUCTION OF SAXS PROFILES FROM PROTEIN STRUCTURES. Excellent review on SAXS. Scatter 2.2b ...
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Protein flexibility linked to severity of Lou Gerhig's disease - The SIBYLS Beamline
http://bl1231.als.lbl.gov/2014/10/protein_flexibility_linked_to_.php
Brought to you by IDAT. Protein flexibility linked to severity of Lou Gerhig's disease. October 21, 2014. SAXS and MX data collected at SIBYLS enabled insights into protein flexibility in one of the proteins involved in Lou Gerigh’s disease. The results were recently published in PNAS and have been highlighted by Today At Berkeley Lab. And and Daily Cal. 8221; Proc. Natl. Acad. Sci. U.S.A. 2014 Oct 14 link. SAXS/PX review in QRB. Protein flexibility linked to severity of Lou Gerhig's disease.
SAXS and EM used to determine structure of Gold Nanopartice - The SIBYLS Beamline
http://bl1231.als.lbl.gov/2014/08/saxs_and_em_used_to_determine_.php
Brought to you by IDAT. SAXS and EM used to determine structure of Gold Nanopartice. August 28, 2014. Structure determination of gold nanoparticles (AuNPs) is necessary for understanding their physical and chemical properties, but only one AuNP larger than 1 nanometer in diameter [a 102-gold atom NP (Au. NP)] has been solved to atomic resolution. Whereas the Au. Read about it here:. 8221; Science 2014 Aug 22 ;345(6199):909-12 link. SAXS/PX review in QRB. SAXS Workshop "Frontiers in biological SAXS".
The SIBYLS Beamline: MX Protocols
http://sibyls.als.lbl.gov/px_protocols
Brought to you by IDAT. The SIBYLS beamline has available a SAM. Tanford Automated Mounting system) style sample mounting robot. We've christened it DOMO. Dynamic Offsite MX Operator). Our storage dewar accepts two of the widely available SSRL 96 sample cassettes, allowing you extended uninterrupted beamtime to screen crystals and collect data. Connecting to the SIBYLS NX Server from Mac/Linux. Connecting to the SIBYLS NX Server from a Windows PC. Data Processing with XDS. Instructions for Sending Samples.
Review of SAXS combined with crystallography and computation - The SIBYLS Beamline
http://sibyls.als.lbl.gov/2007/10/review_of_saxs_combined_with_c.php
Brought to you by IDAT. Review of SAXS combined with crystallography and computation. October 23, 2007. A new review on macromolecular SAXS has been published in the Quarterly Reviews in Biophysics. Putnam, C.D., Hammel, M., Hura, G.L., and Tainer, J.A. The review provides an extensive and up-to-date review on the application of small angle X-ray scattering is available for download. SAXS/PX review in QRB. Protein flexibility linked to severity of Lou Gerhig's disease.
Beamline Usage Policy - The SIBYLS Beamline
http://sibyls.als.lbl.gov/2006/09/beamline_usage_policy.php
Brought to you by IDAT. September 13, 2006. Every person who will use SIBYLS must be trained directly by SIBYLS staff. Users may not operate equipment in the SIBYLS hutch for which they have not been trained. If more then one person will be using the beamline over the course of the assigned time one spokesperson (the original proposal applicant by default) must be present during the entire shift. The spokesperson may be changed by contacting Jane Tanamachi. Up to one week in advance of beamtime. Please i...
SAXS Workshop "Frontiers in biological SAXS" - The SIBYLS Beamline
http://bl1231.als.lbl.gov/2014/06/saxs_workshop_frontiers_in_bio.php
Brought to you by IDAT. SAXS Workshop "Frontiers in biological SAXS". June 20, 2014. We are pleased to announce the 5th annual SIBYLS bioSAXS workshop “Frontiers in biological SAXS”. Date: October 7-8, 2014 Location: Advance Light Source (ALS) at Lawrence Berkeley National Laboratory , Berkeley, CA. Organizers: Michal Hammel, Greg Hura. Tuesday, October 7th LBNL Building 2 , room 100B. 11:30 Lunch at the ALS patio. 12:45 Lois Pollack, Cornell Univesity. 13:15 Edward Snell, Hauptman-Woodward Institute.
Practical High-throughput SAXS - The SIBYLS Beamline
http://bl1231.als.lbl.gov/2014/03/practical_ht_saxs.php
Brought to you by IDAT. March 4, 2014. A new publication is available that describes practical aspects of collecting High-throughput SAXS data at the SIBYLS beamline, with a focus on challenging low concentration samples. Additional practical advice is laid out with respect to interpreting the resulting data. Dyer KN, Hammel M, Rambo RP, Tsutakawa SE, Rodic I, Classen S, Tainer JA, Hura GL. “ High-throughput SAXS for the characterization of biomolecules in solution: a practical approach.
How a Shape-shifting DNA-repair Machine Fights Cancer - The SIBYLS Beamline
http://bl1231.als.lbl.gov/2014/02/rad50_mre11_EMBO_MolCell.php
Brought to you by IDAT. How a Shape-shifting DNA-repair Machine Fights Cancer. February 7, 2014. The SIBYLS beamline was instrumental in providing key structural data for two recent publications exploring the dynamic nature of DNA repair. Read more in the full articles…. Deshpande RA, Williams GJ, Limbo O, Williams RS, Kuhnlein J, Lee J-H, Classen S, Guenther G, Russell P, Tainer JA, Paull TT. “ ATP-driven Rad50 conformations regulate DNA tethering, end resection, and ATM checkpoint signaling.
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Bioisis: welcome - index
Welcome to BIOISIS . the resource for macromolecular SAXS. Splicing factor complex of phosphorylated (P)SF1 residues 1-255 and U2AF65 residues 148-375. Human SF1 ULM-KH-QUA2, which is phosphorylated on Ser80Ser82, bound to U2AF65 RRM1-RRM2-UHM in a splicing factor complex. SAS Tool Box (SASTBX). TAPBPR (TAP Binding Protein, Related). SAXS analysis of Hsp104 wild-type and . RPA DNA-Binding Core with 20mer. RECONSTRUCTION OF SAXS PROFILES FROM PROTEIN STRUCTURES. Excellent review on SAXS. Scatter 2.2b ...
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Thursday, October 22, 2009. Just come across this interesting read. The team carefully chooses the notes such that normal gene expression patterns sound pleasantly in tune, while abnormal data yield discordant sounds.". Imagine listening to 'melody' to decide if things are fine instead of doing lots and lots of experiments to decide whether there is any abnormal gene expression. wow. I really don't mind at all. hihi. Thursday, October 16, 2008. Anyone game enough for protein computer game? Report on nano...
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