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oligonucleotide.de
smFISH » Oligos, Oligonucleotide
http://www.oligonucleotide.de/tag/smfish
Oligonucleotides for single-molecule fluorescence in situ hybridization (smFISH). Kommentare deaktiviert für Oligonucleotides for single-molecule fluorescence in situ hybridization (smFISH). In situ detection methods of RNAs are a valuable and important tool for gene expression analysis. Sunjong Kwon’s mini review gives a short survey on recently developed methods based on fluorescently labeled oligonucleotides. As custom synthesized OligoPool 48. Raj A, van den Boogard P, Rifkin SA, van Oudenaarden A, T...
oligonucleotide.de
biomers.net » Oligos, Oligonucleotide
http://www.oligonucleotide.de/tag/biomers-net
BMN quencher series for qPCR probes. Kommentare deaktiviert für BMN quencher series for qPCR probes. Biomers.net recently introduced two new quencher molecules: BMN-Q590 and BMN-Q650. Together with BMN-Q460 and BMN-Q530 /BMN-Q535 a quencher set spanning the whole relevant spectrum for qPCR probes is now available:. BMN-Q460: Quenching range 400 -530 nm. BMN-Q535: Quenching range 480 -580 nm. BMN-Q590: Quenching range 550 -650 nm. BMN-Q650: Quenching range 550 -750 nm. Tagged with: biomers.net. This new q...
oligonucleotide.de
BMN-Q460 » Oligos, Oligonucleotide
http://www.oligonucleotide.de/tag/bmn-q460
New quencher BMN-Q460 for Molecular Beacons and 5′-nuclease assay probes. Kommentare deaktiviert für New quencher BMN-Q460 for Molecular Beacons and 5′-nuclease assay probes. This new quencher is available for 3′-, 5′- and internal modification of oligonucleotides. Together with the recently introduced BMN-Q530 (replacement for BHQ-1 and IBFQ), biomers.net. Now offers a set of proprietary quenchers covering the wavelength range of 400 nm – 580 nm. Tagged with: biomers.net. 2015 Oligos, Oligonucleotide.
oligonucleotide.de
Oligonucleotides for single-molecule fluorescence in situ hybridization (smFISH) » Oligos, Oligonucleotide
http://www.oligonucleotide.de/oligonucleotides-for-single-molecule-fluorescence-in-situ-hybridization-smfish
Oligonucleotides for single-molecule fluorescence in situ hybridization (smFISH). In situ detection methods of RNAs are a valuable and important tool for gene expression analysis. Sunjong Kwon’s mini review gives a short survey on recently developed methods based on fluorescently labeled oligonucleotides. Kwon S. Single-molecule fluorescence in situ hybridization: Quantitative imaging of single RNA molecules. BMB Reports 2013; 46(2): 65-72. As custom synthesized OligoPool 48. New quencher BMN-Q460 for Mo...
oligonucleotide.de
OligoPool » Oligos, Oligonucleotide
http://www.oligonucleotide.de/tag/oligopool
Oligonucleotides for single-molecule fluorescence in situ hybridization (smFISH). Kommentare deaktiviert für Oligonucleotides for single-molecule fluorescence in situ hybridization (smFISH). In situ detection methods of RNAs are a valuable and important tool for gene expression analysis. Sunjong Kwon’s mini review gives a short survey on recently developed methods based on fluorescently labeled oligonucleotides. As custom synthesized OligoPool 48. Raj A, van den Boogard P, Rifkin SA, van Oudenaarden A, T...
oligonucleotide.de
Oligonucleotid-Peptid-Konjugate » Oligos, Oligonucleotide
http://www.oligonucleotide.de/oligonucleotid-peptid-konjugate
In Oligonucleotid-Peptid-Konjugaten sind Peptide kovalent an Oligonucleotide geknüpft. Diese Konstrukte gewinnen in unterschiedlichen Anwendungen zunehmend an Bedeutung, beispielsweise bei Antisense Oligonucleotiden zur besseren Zellaufnahme des Oligos, als Aptamere oder in Enzym- bzw. Antikörper-Assays. Eine Direktsynthese von Oligonucleotid-Peptid-Konjugaten ist in den allermeisten Fällen nicht möglich, da die üblichen Schutzgruppenstrategien von Oligonucleotid- und Peptidsynthese nicht oder nur sehr b...
oligonucleotide.de
BMN-Q530 » Oligos, Oligonucleotide
http://www.oligonucleotide.de/tag/bmn-q530
Kommentare deaktiviert für DoubleQuenched Probes. Bei relativ langen qPCR Probes kann sich das Signal-Rausch-Verhältnis verschlechtern, da Fluorophor und Quencher weit voneinander getrennt sind und dadurch die Fluoreszenz nicht optimal gelöscht wird. Der Einbau eines weiteren Quenchers in der Nähe des 5′-Fluorophors kann hier Abhilfe schaffen. QPCR Probes mit BMN-Q530 Quencher. Kommentare deaktiviert für qPCR Probes mit BMN-Q530 Quencher. Ein neuer Quencher im Programm von biomers.net.
oligonucleotide.de
Oligonucleotide » Oligos, Oligonucleotide
http://www.oligonucleotide.de/tag/oligonucleotide
Literature highlight: Smart-seq2 protocol. Kommentare deaktiviert für Literature highlight: Smart-seq2 protocol. Simone Picelli, Omid R Faridani, Åsa K Björklund, Gösta Winberg, Sven Sagasser and Rickard Sandberg, Full-length RNA-seq from single cells using Smart-seq2,. Describe a detailed protocol with improved sensitivity and accuracy for transcriptome analysis from single cells comprising the generation of full-length cDNA and sequencing libraries. Oligonucleotides are available from biomers.net.
oligonucleotide.de
Smart-seq » Oligos, Oligonucleotide
http://www.oligonucleotide.de/tag/smart-seq
Literature highlight: Smart-seq2 protocol. Kommentare deaktiviert für Literature highlight: Smart-seq2 protocol. Simone Picelli, Omid R Faridani, Åsa K Björklund, Gösta Winberg, Sven Sagasser and Rickard Sandberg, Full-length RNA-seq from single cells using Smart-seq2,. Describe a detailed protocol with improved sensitivity and accuracy for transcriptome analysis from single cells comprising the generation of full-length cDNA and sequencing libraries. Oligonucleotides are available from biomers.net.
