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C.T.L ELISPOT細胞激素檢測系統
Thursday, September 15, 2005. CTL ELISPOT Assays for Cells That Secrete Cytokines. ELISPOT Enzyme-linked immunospot 最早是被發展來偵測分泌特定抗體的細胞，目前ELISPOT已經被廣泛用來偵測某些製造並分泌特定反應因子的細胞，這些反應因子包含細胞激素 Cytokine 、趨化激素 Chemokine 或是顆粒酵素 Granzyme。 ELISPOT方法操作簡單，而且能提供非常類似於體內實驗的環境 ex vivo ，對於測量週邊血或其中淋巴球的免疫功能而言，是一項功能強大的工具。 本公司生物科學部研發出ELISPOT檢測試劑是在披覆PVDF膜的培養96微孔盤上，吸附上經純化、且無毒性 不含sodium azide、內毒素endotoxin 的抗體。 最後可以使用傳統方法以顯微鏡進行人工判讀，或使用高效能之影像分析系統 ELISPOT Plate Reader 進行判讀。 提示 前兩天的實驗步驟中，必須在無菌操作櫃中使用 ELISPOT 組套與試劑，並用無菌技術處理細胞。 提示 Kit Prot...
