crr.columbia.edu

raraf.org

Fast Neutrons

http://www.raraf.org/neutronsfast.html

Fast Neutrons at RARAF. Even lower-energy neutron beams (. Large numbers of 14 MeV neutrons can be produced using the T(d,n). He reaction. The neutron energy, fluence, and dose rate are nearly independent of angle so that planning irradiations and designing fixtures to hold samples are relatively easy. A significant fraction of the energy deposited in tissue by 14 MeV neutrons is from alpha particles and heavy-ion recoils. Approximately 70% of the energy deposition is from proton recoils. Although the ta...

raraf.org

RARAF: Biological Systems

http://www.raraf.org/bsystems.html

Our primary irradiation method on our microbeam are cells in culture dishes. this is a brief overview of our culture system. We have irradiated skin models looking at long range effects from radiation. We are able to irradiate C. elegans. Nematodes in a high throughput manner using a microbeam. We have irradiated medaka embryos and are expanding our capabilities to irradiate Zebrafish. We have taken advantage of mouse ear geometries to microbeam irradiate specific cells and structures in the ears.

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Track Segment

http://www.raraf.org/tracksegment.html

Track Segment Charged-Particle Irradiator. In a track-segment irradiation, an initially monoenergetic beam of charged particles passes through the thin sample ( 10. M) so that the same segments of the particle tracks are deposited in all the material of interest. Particles with linear energy transfer (LET) between 10 and 200 keV/ m. M are available utilizing beams of protons, deuterons, helium-3, and helium-4 ions as listed below. Charged-Particle Beams for Radiobiology. Track-segment irradiations and do...

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Cell Cultures

http://www.raraf.org/cellculture.html

Cell Culture Dishes for Microbeam Irradiation. Standard microbeam cell culture dish. We have drafted instruction sheets on preparing microbeam dishes. And plating cells for microbeam irradiation. A list of cell lines that have been previously used for microbeam irradiation is available here. The dish making and handling procedures have been demonstrated in a video for our microbeam training course. Video for Dish Handling. Radiological Research Accelerator Facility Nevis Laboratories.

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What is a Microbeam?

http://www.raraf.org/whatismicrobeam.html

What is a Microbeam? What is a Microbeam? The main components of the microbeam. A source of radiation. A focusing system [ More. An ion detector for determining delivered dose [ More. An imaging system for detecting the target of interest [ More. A stage for moving the target of interest into the beam and/or a beam deflector for moving the beam to the target. Microbeam techniques can deliver exactly one or more particles per cell. Irradiation of Cells in Precise Spatial or Temporal Locations. Between ene...

raraf.org

Permanent Magnet Microbeam

http://www.raraf.org/pmm.html

What is a Microbeam? The permanent magnet microbeam (PMM), under development at RARAF, presents an alternative approach to microbeam design. Instead of focusing the ion beam using electromagnetic or electrostatic lenses, this system uses permanent magnets, which require no power supplies. Two permanent magnet quadrupole triplets have been purchased from STI Optronics. Just prior to the decommissioning of the RARAF Van de Graaff accelerator in June 2005, we had attained a beam spot size of 20 µm. As a...

raraf.org

X-ray Microbeam

http://www.raraf.org/xrays.html

What is a Microbeam? We are upgrading the RARAF microbeam to include soft x rays: characteristic Kα x rays from Ti, 4.5 keV (higher energies are not feasible due to Compton scattering effects). The x-ray microbeam (left) is mounted on the end of a horizontal beam line on the first floor of RARAF. Because the x rays are being produced by reflection instead of transmission, the x-ray beam will be vertical. Radiological Research Accelerator Facility Nevis Laboratories.

raraf.org

Neutron Microbeam

http://www.raraf.org/neutrons.html

What is a Microbeam? Schematic of the neutron microbeam. The proton microbeam is incident on a lithium target, and neutrons are produced with a highly forward-peaked angular distribution. Predicted neutron yields, microbeam diameters, neutron energies, and dose rates for different proton energies. Selected proton energy and characteristics of the predicted neutron microbeam are highlighted. H ion emitted from the. Radiological Research Accelerator Facility Nevis Laboratories.

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Microbeam Online Imaging

http://www.raraf.org/onlineimaging.html

RARAF Imaging Capabilities on the Microbeam II Charged-Particle Microbeam End Station. Cells with multiple parts labeled with separate fluorophores. DNA (blue), mitochondria (red) and Actin fibers (green) are shown here. Multiphoton microscope image acquired during an irradiation experiment using mitochondria sites (green) as targets. The cross-hairs (red) mark the center of the image, which coincides with the position of the ion beam. More information about out Multi-Photon Imaging is available here.