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From Disease Genes to Protein Pathways :: DiGtoP

DiGtoP is a research consortium that has been formed to develop standardized methodology to place disease genes into pathways, building upon recent advances into genomics and proteomics. Studying physical interactions between proteins, complemented by reciprocal validation and functional studies, will establish a mammalian proteomic database relevant to disease pathways and therapy and a pipeline for study of novel disease genes as they are discovered.

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DiGtoP is a research consortium that has been formed to develop standardized methodology to place disease genes into pathways, building upon recent advances into genomics and proteomics. Studying physical interactions between proteins, complemented by reciprocal validation and functional studies, will establish a mammalian proteomic database relevant to disease pathways and therapy and a pipeline for study of novel disease genes as they are discovered.
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From Disease Genes to Protein Pathways :: DiGtoP | digtop.de Reviews

https://digtop.de

DiGtoP is a research consortium that has been formed to develop standardized methodology to place disease genes into pathways, building upon recent advances into genomics and proteomics. Studying physical interactions between proteins, complemented by reciprocal validation and functional studies, will establish a mammalian proteomic database relevant to disease pathways and therapy and a pipeline for study of novel disease genes as they are discovered.

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From Disease Genes to Protein Pathways :: DiGtoP

http://www.digtop.de/tools/whydigtop.php

Transient overexpression or tagged cell lines? The workhorse of cell regulation studies has been the transient overexpression of proteins of interest. Massive overproduction of a protein runs the risk that it enters incorrect cell compartments and now interacts with proteins that it never normally contacts. Thus, inferring cellular location is risky, while overexpression should never be used to provide material for proteomics studies. Features of Cell Regulation / Effect on Experiment.

2

From Disease Genes to Protein Pathways :: DiGtoP

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PANTHER - Protein Analysis Through Evolutionary Relationships. HPRD - Human Protein Reference Database. HUPO - Human Proteome Organisation. PIR - Protein Information Ressource. PSI - Protein Structure Initiative. DIP - Database of Interacting Proteins. MIPS Protein-Protein Interaction Database. SCOPPI - Structural Classification of Protein-Protein Interfaces. STITCH - Chemical-Protein Interactions. Phospho.ELM - Curated Database of Phosphorylation Sites. ELM - Database of Regulatory Motifs.

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From Disease Genes to Protein Pathways :: DiGtoP

http://www.digtop.de/projects/overview.php

Putting the genes into networks. For each chosen gene we will:. Tag the protein at either N or C terminus, or both, by recombination into the endogenous gene or BAC transgenesis to ensure that expression is physiological from the endogenous regulatory elements. Because the main tag includes GFP, the subcellular localization will be documented in living cells throughout the cell cycle using an HT process that is already established. Determine the interaction partners by mass spectrometry. Evaluation of pr...

4

From Disease Genes to Protein Pathways :: DiGtoP

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Frank Schnütgen, Franziska Ehrmann, Ina Poser, Nina C Hubner, Jens Hansen, Thomas Floss, Ingrid deVries, Wolfgang Wurst, Anthony Hyman, Matthias Mann, Harald von Melchner (2011) Resources for proteomics in mouse embryonic stem cells. Nature Methods. Gibson TJ (2009) Cell regulation: determined to signal discrete cooperation. Trends Biochem Sci. Elefsinioti A, Ackermann M, Beyer A (2009) Accounting for redundancy when integrating gene interaction databases. PLoS one. Theis M, Slabicki M, Junqueira M, Pasz...

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From Disease Genes to Protein Pathways :: DiGtoP

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SyBoSS - Systems Biology of Stem Cells and Reprogramming: Our Partners

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Go to the homepage. Skip to the navigation. Skip to the content. Skip to the sidebar. Go to the imprint. SyBoSS - Systems Biology of Stem Cells and Reprogramming. Supported by the EU Seventh Research Framework Programme (FP7). Recombineering is DNA engineering based on the use of homologous recombination in E. coli. The Stewart lab first showed that the phage proteins RecE/RecT and the Red operon of lambda phage are ideally suited for many DNA engineering tasks. To be updated soon. And eukaryotic cells (...

vonmelchner.de vonmelchner.de

Molecular Hematology, University of Frankfurt Medical School

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Within the framework of the International Mouse Knock Out Consortium. IKMC), my laboratory participates in the systematic knock-out of mouse genes in ESCs using high throughput sequence-based approaches, such as gene trapping and gene targeting. The overall goal of the effort is to assemble a library of ES cell lines harboring conditional mutations in every single gene of the mouse genome, and to make these cell lines available to the scientific community for production of mutant mice. The Laboratory is ...

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From Disease Genes to Protein Pathways :: DiGtoP

DiGtoP is a research consortium that has been formed to develop standardized methodology to place disease genes into pathways, building upon recent advances into genomics and proteomics. Studying physical interactions between proteins, complemented by reciprocal validation and functional studies, will establish a mammalian proteomic database relevant to disease pathways and therapy and a pipeline for study of novel disease genes as they are discovered.

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