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Tuesday, March 18, 2008. Testing whether the CRP-S sequence confers Sxy-dependence. I am currently studying how CRP binds to and activates transcription from promoters with CRP-S sites. These promoters are unique in H. influenzae. For two reasons: 1. They depend on the Sxy protein for transcription, and 2. The CRP-binding sites (called CRP-S ) in these promoters differ at the sequence level from other CRP sites (called CRP-N ) in the genome. Promoter and then by mutating the E. coli lacZ. Promoter mutant...

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duckfeet | duckinthemuck.blogspot.com Reviews

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Tuesday, March 18, 2008. Testing whether the CRP-S sequence confers Sxy-dependence. I am currently studying how CRP binds to and activates transcription from promoters with CRP-S sites. These promoters are unique in H. influenzae. For two reasons: 1. They depend on the Sxy protein for transcription, and 2. The CRP-binding sites (called CRP-S ) in these promoters differ at the sequence level from other CRP sites (called CRP-N ) in the genome. Promoter and then by mutating the E. coli lacZ. Promoter mutant...

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5 43 comments

6 promoter

7 key point

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duckfeet | duckinthemuck.blogspot.com Reviews

https://duckinthemuck.blogspot.com

Tuesday, March 18, 2008. Testing whether the CRP-S sequence confers Sxy-dependence. I am currently studying how CRP binds to and activates transcription from promoters with CRP-S sites. These promoters are unique in H. influenzae. For two reasons: 1. They depend on the Sxy protein for transcription, and 2. The CRP-binding sites (called CRP-S ) in these promoters differ at the sequence level from other CRP sites (called CRP-N ) in the genome. Promoter and then by mutating the E. coli lacZ. Promoter mutant...

INTERNAL PAGES

duckinthemuck.blogspot.com

1

duckfeet: August 2007

http://duckinthemuck.blogspot.com/2007_08_01_archive.html

Sunday, August 26, 2007. Happy to have been wrong about HiCRP. The second satisfaction came from discovering that, contrary to earlier conclusions, H. influenzae. CRP (HiCRP) appears to have a very high affinity for DNA, comperable to that of E. coli. Posted by Some call me Tim @ 7:02 PM. Monday, August 20, 2007. Posted by Some call me Tim @ 7:00 PM. Sunday, August 12, 2007. Using bandshifts to study CRP-DNA interactions. Posted by Some call me Tim @ 9:01 PM. Tuesday, August 07, 2007. Can cause similarly...

2

duckfeet: August 2006

http://duckinthemuck.blogspot.com/2006_08_01_archive.html

Monday, August 28, 2006. And, sadly, after an exhausting day I'm not going to post much to make up for it. Tomorrow is dedicated to composing pieces of our sxy manuscript, including: crunching today's data, getting figures together, writing my and Laura's Material and Methods sections, finishing the strain list, and (time premitting, which I doubt) thinking about elements of the discussion. Posted by Some call me Tim @ 7:24 PM. Wednesday, August 23, 2006. The results are in: sxy. Monday, August 21, 2006.

3

duckfeet: More results from Sunita and Andrew

http://duckinthemuck.blogspot.com/2007/11/more-results-from-sunita-and-andrew.html

Sunday, November 11, 2007. More results from Sunita and Andrew. Our results that suggest Sxy binds to DNA are exciting but suspicious; all Sxy-DNA binding data can be explained by the presence of contaminating CRP in the Sxy protein preps. This is because the Sxy-DNA binding data is identical to CRP-DNA data. First, EcSxy binds DNA but HiSxy does not. Second, EcSxy greatly prefers the pilA-. N (CRP-N mutant) site over the wildtype pilA. The next set of experiments is clear: 1. If tomorrow’s experiments s...

4

duckfeet: December 2006

http://duckinthemuck.blogspot.com/2006_12_01_archive.html

Wednesday, December 20, 2006. A note on today. Hey Blog. Today I am composing responses to questions that have arisen in Rosie's current grant proposal. I will also describe some experiments that may be of use in the proposal. Posted by Some call me Tim @ 9:51 AM. Sunday, December 03, 2006. Posted by Some call me Tim @ 6:36 PM. Some call me Tim. View my complete profile. Testing whether the CRP-S sequence confers Sxy-dep. Why CRP-S sites are special. The complexities of gene regulation in E. coli.

5

duckfeet: The complexities of gene regulation in E. coli

http://duckinthemuck.blogspot.com/2007/12/complexities-of-gene-regulation-in-e.html

Friday, December 28, 2007. The complexities of gene regulation in E. coli. We are currently identifying genes that belong to the Sxy regulon in E. coli. The only other well-characterized Sxy regulon was identified by our work in H. influenzae. S genome is over 2-times larger than H. influenzae. S and, not surprisingly, the E. coli. Sxy regulon contains more genes. The E. coli. This additional complexity is a consequence of lifestyle. E. coli. Is a more versatile organism than H. influenzae. Which lives i...

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Tuesday, March 18, 2008. Testing whether the CRP-S sequence confers Sxy-dependence. I am currently studying how CRP binds to and activates transcription from promoters with CRP-S sites. These promoters are unique in H. influenzae. For two reasons: 1. They depend on the Sxy protein for transcription, and 2. The CRP-binding sites (called CRP-S ) in these promoters differ at the sequence level from other CRP sites (called CRP-N ) in the genome. Promoter and then by mutating the E. coli lacZ. Promoter mutant...

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