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Ethan-omics | Everything Ethan knows about biology – The Ethan-ome

Everything Ethan knows about biology - The Ethan-ome

http://ethanomics.wordpress.com/

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Ethan-omics | Everything Ethan knows about biology – The Ethan-ome | ethanomics.wordpress.com Reviews

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Everything Ethan knows about biology - The Ethan-ome

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Ribo-Zero rRNA removal for RNA-seq with FFPE samples | Ethan-omics

https://ethanomics.wordpress.com/2012/09/20/ribo-zero-rrna-removal-for-rna-seq-with-ffpe-samples

Everything Ethan knows about biology – The Ethan-ome. Ribo-Zero rRNA removal for RNA-seq with FFPE samples. September 20, 2012. Take a look at the DESeq output. Pretty clean. I was a little surprised. BTW, that is the QC report that ezDESeq.sh makes for you. All you have to do is make two folders with your fastq files and run the script. It’s that easy. Finally, here was my adaptation of the Ribo-Zero protocol. So there is no reason to buy another expensive reagent to precipitate your RNA. 8212;“I ...

2

Gibson Assembly Master Mix | Ethan-omics

https://ethanomics.wordpress.com/2013/03/01/gibson-assembly-master-mix

Everything Ethan knows about biology – The Ethan-ome. Gibson Assembly Master Mix. March 1, 2013. As far as the Master Mix, you can buy it from NEB or you can save money and buy all the components separately and save some money. It didn’t really make sense to me the way others were doing it so I recalculated everything and am doing it as described here: Gibson Assembly Master Mix. It worked better then the NEB master mix, but I have no idea why. As far as primer design, this is how it goes for me:. Gibson...

3

Homemade AMPure XP beads | Ethan-omics

https://ethanomics.wordpress.com/homemade-ampure-xp-beads

Everything Ethan knows about biology – The Ethan-ome. Homemade AMPure XP beads. Homemade AMPure XP beads. Great Work, I will test this soon. Do you have any idea how long time the beads can last after preparation? Is it only the 1 month you state as interval between testing or do you think one can go longer? Homemade AMPure XP beads « Ethan-omics. Leave a Reply Cancel reply. Enter your comment here. Fill in your details below or click an icon to log in:. Address never made public). Join 49 other followers.

4

Some good comments on ChIP-seq library prep | Ethan-omics

https://ethanomics.wordpress.com/2013/04/09/some-good-comments-on-chip-seq-library-prep

Everything Ethan knows about biology – The Ethan-ome. Some good comments on ChIP-seq library prep. April 9, 2013. Well I suspected the Illumina ChIP-seq kit wasn’t very good. Well here is what someone else had to say about it and some other good comments on making ChIP-seq library preps. 1) The SYBR Gold gel protocol is madness! 4) I only do a single post-ligation Ampure XP clean-up – primarily for speed – and it works fine, but also to reduce the risk of losing LMW DNA. I have never seen...5) I use Qiag...

5

BsaI – Worst restriction enzyme ever | Ethan-omics

https://ethanomics.wordpress.com/2013/06/28/bsai-worst-restriction-enzyme-ever

Everything Ethan knows about biology – The Ethan-ome. BsaI – Worst restriction enzyme ever. June 28, 2013. This is public service announcement. BsaI is the worst restriction enzyme ever. Never use it or any protocol that suggests you use it. I know that includes just about every TALE construction protocol, but really save yourself a bunch of pain and suffering and don’t use BsaI. From → Posts. Maybe that is where some users go astray. Good luck. Here are a couple posts from a TALE Google Group:. 8220;Bsa...

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listerlab.org listerlab.org

Lister Lab - The University of Western Australia

http://www.listerlab.org/people.html

Genetic and epigenomic variation in clonal plants. DNA methylation dynamics in the plant genome. Stem cell epigenomics, epigenome manipulation. Developing gene regulatory circuits for controlling gene expression in plants. Forrest Research Foundation PhD scholar. Genome and epigenome dynamics in brain, bioinformatics. Evolution of DNA methylation systems. Forrest Research Foundation PhD Scholar. Cell type resolution plant epigenomics. Control of DNA methylation patterns in the plant genome.

listerlab.org listerlab.org

Lister Lab - The University of Western Australia

http://www.listerlab.org/links.html

AMATA - The Australasian Genomic Technologies Association. NIH Roadmap Epigenomics Project. ARC CoE Plant Energy Biology. Computational Systems Biology CoE. The Centre for Integrative Bee Research. The University of Western Australia. School of Chemistry and Biochemistry. ARC CoE Plant Energy Biology. Computational Systems Biology CoE. The Centre for Integrative Bee Research. The University of Western Australia. School of Chemistry and Biochemistry. Ph: 61 8 6488 4407. The University of Western Australia.

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Ethan-omics | Everything Ethan knows about biology – The Ethan-ome

Everything Ethan knows about biology – The Ethan-ome. November 18, 2015. How to retrieve something (box, cryovial, etc.) from a liquid nitrogen tank. Mostly I just want to thank Brian. Your Nobel Prize worthy blog post. Saved me big time. February 19, 2014. MeDIP-seq data analysis – diffReps. After sitting on the data for longer then anyone should sit on data (about a year), a new ChIP-seq analysis package called diffReps. June 28, 2013. BsaI – Worst restriction enzyme ever. April 9, 2013. Well I suspect...

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