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Brewer/Raghuraman Lab Home Page

Smash-&-grab yeast DNA prep. NIB-n-grab yeast DNA prep for 2-D gels. Genomic yeast DNA for 2-D gels. Yeast DNA in agarose plugs. YACs for yeast transformation. L waltii genome browser. Welcome to the lab page of the Brewer/Raghuraman lab. We use genomic tools and the budding yeast. To investigate the regulation of eukaryotic chromosomal DNA replication- how cells choose which origins of replication to use, how they decide when in S phase to fire those origins, and how cells respond to replication stress.

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Brewer/Raghuraman Lab Home Page | fangman-brewer.genetics.washington.edu Reviews
<META>
DESCRIPTION
Smash-&-grab yeast DNA prep. NIB-n-grab yeast DNA prep for 2-D gels. Genomic yeast DNA for 2-D gels. Yeast DNA in agarose plugs. YACs for yeast transformation. L waltii genome browser. Welcome to the lab page of the Brewer/Raghuraman lab. We use genomic tools and the budding yeast. To investigate the regulation of eukaryotic chromosomal DNA replication- how cells choose which origins of replication to use, how they decide when in S phase to fire those origins, and how cells respond to replication stress.
<META>
KEYWORDS
1 protocols
2 dna preps
3 2 d gels
4 standard 2 d gels
5 fork direction gels
6 replication timing
7 density transfer
8 comparative hybridization
9 transient hemimethylation
10 transformation
CONTENT
Page content here
KEYWORDS ON
PAGE
protocols,dna preps,2 d gels,standard 2 d gels,fork direction gels,replication timing,density transfer,comparative hybridization,transient hemimethylation,transformation,frozen competent yeast,yacs for transformation,fluctuation analysis,resources,people
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Brewer/Raghuraman Lab Home Page | fangman-brewer.genetics.washington.edu Reviews

https://fangman-brewer.genetics.washington.edu

Smash-&-grab yeast DNA prep. NIB-n-grab yeast DNA prep for 2-D gels. Genomic yeast DNA for 2-D gels. Yeast DNA in agarose plugs. YACs for yeast transformation. L waltii genome browser. Welcome to the lab page of the Brewer/Raghuraman lab. We use genomic tools and the budding yeast. To investigate the regulation of eukaryotic chromosomal DNA replication- how cells choose which origins of replication to use, how they decide when in S phase to fire those origins, and how cells respond to replication stress.

INTERNAL PAGES

fangman-brewer.genetics.washington.edu fangman-brewer.genetics.washington.edu
1

Brewer/Raghuraman Lab Home Page

http://fangman-brewer.genetics.washington.edu/index.html

Smash-&-grab yeast DNA prep. NIB-n-grab yeast DNA prep for 2-D gels. Genomic yeast DNA for 2-D gels. Yeast DNA in agarose plugs. YACs for yeast transformation. L waltii genome browser. Welcome to the lab page of the Brewer/Raghuraman lab. We use genomic tools and the budding yeast. To investigate the regulation of eukaryotic chromosomal DNA replication- how cells choose which origins of replication to use, how they decide when in S phase to fire those origins, and how cells respond to replication stress.

2

b-r lab template

http://fangman-brewer.genetics.washington.edu/hemimethylation.html

Smash-&-grab yeast DNA prep. NIB-n-grab yeast DNA prep for 2-D gels. Genomic yeast DNA for 2-D gels. Yeast DNA in agarose plugs. YACs for yeast transformation. L waltii genome browser. Replication timing using transient hemimethylation. Kathy Friedman's protocol; see Friedman, K. L., M. K. Raghuraman, W. L. Fangman, and B. J. Brewer (1995). Analysis of the temporal program of replication initiation in yeast chromosomes. J. Cell Sci. Suppl. Rationale of methylation timing approach. Hold at 37 C for 120 mi...

3

B-R lab publications

http://fangman-brewer.genetics.washington.edu/publications.html

Smash-&-grab yeast DNA prep. NIB-n-grab yeast DNA prep for 2-D gels. Genomic yeast DNA for 2-D gels. Yeast DNA in agarose plugs. YACs for yeast transformation. L waltii genome browser. Selected publications from the Brewer/Raghu lab. Peng, J., Raghuraman, M.K., and Feng, W. (2014). Analysis of replication timing using synchronized budding yeast cultures. Methods Mol Biol 1170, 477-499. Payen, C., Di Rienzi, S.C., Ong, G.T., Pogachar, J.L., Sanchez, J.C., Sunshine, A&#4...G3: Genes, Genomes, Genetics.

4

2-D gels

http://fangman-brewer.genetics.washington.edu/2Dgel.html

Smash-&-grab yeast DNA prep. NIB-n-grab yeast DNA prep for 2-D gels. Genomic yeast DNA for 2-D gels. Yeast DNA in agarose plugs. YACs for yeast transformation. L waltii genome browser. 2-D Agarose Gel Electrophoresis of DNA Replication Intermediates. Bonny Brewer's protocol; for an in-depth review of the method, see Friedman, K. and Brewer, B. 1995) Analysis of replication intermediates by two-dimensional agarose gel electrophoresis. Meth. Enzymol. 2001, Science 294; 115-121). Avoid using spermidine in r...

5

Fluctuation analysis

http://fangman-brewer.genetics.washington.edu/fluctuation.html

Smash-&-grab yeast DNA prep. NIB-n-grab yeast DNA prep for 2-D gels. Genomic yeast DNA for 2-D gels. Yeast DNA in agarose plugs. YACs for yeast transformation. L waltii genome browser. Determination of Mutation Rate by Fluctuation Analysis. Adapted by Seung-Been Steven Lee from the. Protocol of Greg Lang and based on the original Luria-Delbruck bacterial protocol. Last updated: November 2013. Determination of Mutation Rate using Fluctuation Assay. Greg Lang (personal communication). The basic idea of the...

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gardnerlab.org gardnerlab.org

Gardner Lab | Understanding Ubiquitin's Functions

http://www.gardnerlab.org/Protocols.html

How they do it. Bacterial Competent Cells and Transformation. Bacterial Electrocompetent Cells and Electroporation. SDS-PAGE and Western Blotting. Coomassie Staining SDS-PAGE Gels. Silver Staining SDS-PAGE Gels. GST Fusion Protein Purification. His-Tag Fusion Protein Purification. TAP-Tag Fusion Protein Purification. Coupling Antibodies to Protein A or G. Rapid Yeast Genomic Prep. Quick Purification of DNA from Agarose Gels. Big Dye Automated Sequencing.

dunham.gs.washington.edu dunham.gs.washington.edu

Dunham Lab > People

http://dunham.gs.washington.edu/people.shtml

Lab Members and Collaborators. Current Members of the Lab. Ivan Liachko, Postdoc. Emily Mitchell, Lab Manager. Elyse Hope, GS Graduate Student. Monica Sanchez, MCB Graduate Student. Noah Hanson, Research Staff (previously Undergraduate Researcher and Mary Gates Scholar). Caiti Heil, Postdoc. Clara Amorosi, GS Graduate Student. Abby Keller, MCB Graduate Student. Bryce Taylor, Postdoc. Anja Ollodart, MCB Graduate Student. Erica Alcantara, Undergraduate Researcher. Kolena Dang, Undergraduate Researcher.

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Brewer/Raghuraman Lab Home Page

Smash-&-grab yeast DNA prep. NIB-n-grab yeast DNA prep for 2-D gels. Genomic yeast DNA for 2-D gels. Yeast DNA in agarose plugs. YACs for yeast transformation. L waltii genome browser. Welcome to the lab page of the Brewer/Raghuraman lab. We use genomic tools and the budding yeast. To investigate the regulation of eukaryotic chromosomal DNA replication- how cells choose which origins of replication to use, how they decide when in S phase to fire those origins, and how cells respond to replication stress.

fangman-brewer.genetics.washington.edu fangman-brewer.genetics.washington.edu

Brewer/Raghuraman Lab Home Page

Smash-&-grab yeast DNA prep. NIB-n-grab yeast DNA prep for 2-D gels. Genomic yeast DNA for 2-D gels. Yeast DNA in agarose plugs. YACs for yeast transformation. L waltii genome browser. Welcome to the lab page of the Brewer/Raghuraman lab. We use genomic tools and the budding yeast. To investigate the regulation of eukaryotic chromosomal DNA replication- how cells choose which origins of replication to use, how they decide when in S phase to fire those origins, and how cells respond to replication stress.

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