flycrispr.molbio.wisc.edu

Overview | flyCRISPR

Skip to main content. Recognizing the potential of harnessing this system for precise genome engineering in other organisms, Jinek and colleagues identified a minimal two-component system required for the site-specific cleavage of DNA: Cas9 and a chimeric RNA (chiRNA) comprising the crRNA and tracrRNA from S. pyogenes (JINEK et al. 2012). Thus, in this modified CRISPR RNA/Cas9 system a common nuclease is directed to specific DNA sequences by a short, readily generated RNA. As they become available. 2013 ...

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Overview | flyCRISPR | flycrispr.molbio.wisc.edu Reviews

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Skip to main content. Recognizing the potential of harnessing this system for precise genome engineering in other organisms, Jinek and colleagues identified a minimal two-component system required for the site-specific cleavage of DNA: Cas9 and a chimeric RNA (chiRNA) comprising the crRNA and tracrRNA from S. pyogenes (JINEK et al. 2012). Thus, in this modified CRISPR RNA/Cas9 system a common nuclease is directed to specific DNA sequences by a short, readily generated RNA. As they become available. 2013 ...

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2 o'connor giles lab

3 wildonger lab

4 harrison lab

5 main menu

6 overview

7 scarless ge

8 reagents

9 protocols

10 tools

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Overview | flyCRISPR | flycrispr.molbio.wisc.edu Reviews

https://flycrispr.molbio.wisc.edu

Skip to main content. Recognizing the potential of harnessing this system for precise genome engineering in other organisms, Jinek and colleagues identified a minimal two-component system required for the site-specific cleavage of DNA: Cas9 and a chimeric RNA (chiRNA) comprising the crRNA and tracrRNA from S. pyogenes (JINEK et al. 2012). Thus, in this modified CRISPR RNA/Cas9 system a common nuclease is directed to specific DNA sequences by a short, readily generated RNA. As they become available. 2013 ...

SUBDOMAINS

tools.flycrispr.molbio.wisc.edu

CRISPR Optimal Target Finder

Genome release 6 (the current FlyBase version) is now available for target design! Enter genomic DNA sequence to find CRISPR target sites:. Omit header lines and special characters.). Drosophila melanogaster (r 5/dm3). Drosophila melanogaster (r 6). Drosophila mauritiana (Dmau MS17). Drosophila ananassae (dana r1.3 FB2011 07). Drosophila erecta (dere r1.3 FB2011 08). Drosophila persimilis (dper r1.3 FB2010 02). Drosophila pseudoobscura (dpse r3.1 FB2013 03). Drosophila mojavensis (dmoj r1.3 FB2011 05).

INTERNAL PAGES

flycrispr.molbio.wisc.edu

1

Reagents | flyCRISPR

http://www.flycrispr.molbio.wisc.edu/reagents

Skip to main content. The following CRISPR/Cas9 fly lines are available through the Bloomington Drosophila Stock Center. 51321 w[1118]; PBac{y[ mDint2] w[GMR.PHb]=U6-tracrRNA}VK00037/CyO, P{Wee-P.ph0}2. 51322 y[1] M{w[GMR.PHb]=U6-tracrRNA}ZH-2A w[1118]/FM7c, P{w[ mC]=GAL4-Kr.C}DC1, P{w[ mC]=UAS-GFP.S65T}DC5, sn. 51323 y[1] M{vas-Cas9}ZH-2A w[1118]/FM7c. 51324 w[1118]; PBac{y[ mDint2]=vas-Cas9}VK00027. 51325 w[1118]; PBac{y[ mDint2]=vas-Cas9,U6-tracrRNA}VK00027. PHD-2xHA-ScarlessDsRed (donor vector for sc...

2

Tools | flyCRISPR

http://www.flycrispr.molbio.wisc.edu/tools

Skip to main content. FlyCRISPR Optimal Target Finder. Is a web tool for identifying CRISPR target sites and evaluating their specificity using transparent rules based on empirical data in the literature so you can easily select the best target(s) for your project. The rules behind our algorithms are detailed in the user manual. And will be updated as new data becomes available. 4 Hsu, P.D. et al. DNA targeting specificity of RNA-guided Cas9 nucleases. Nat Biotechnol (2013). 6 Pattanayak, V. et al&#4...

3

Protocols - Links | flyCRISPR

http://www.flycrispr.molbio.wisc.edu/protocols

Skip to main content. Click on the links below for detailed protocols. Download a composite PDF of all protocols. 2013 Board of Regents of the University of Wisconsin System.

4

News & Results | flyCRISPR

http://www.flycrispr.molbio.wisc.edu/news

Skip to main content. Visit the flyCRISPR Discussion Group. For ongoing discussions. We'll also be posting new data and reagents here as they become available. June 10, 2015. Tools for scarless HDR with screenable markers. HDR with dsDNA donors offers mutliple advantages, including the ability to edit over large regions, insert large exogneous sequences, and incorporate selection markers. While selectable markers greatly facilitate identification of engineered organisms, most methods for removing mar...

5

Links & References | flyCRISPR

http://www.flycrispr.molbio.wisc.edu/links

Skip to main content. Drosophila CRISPR target finders:. FlyCRISPR Optimal Target Finder. Zhang lab CRISPR website. Joung lab CRISPR website. Goldstein lab CRISPR website. Bassett, A.R., and Liu, J.L. (2014). CRISPR/Cas9 and Genome Editing in Drosophila. J Genet Genomics. Bassett, A.R., Tibbit, C., Ponting, C.P., and Liu, J.L. (2014). Mutagenesis and homologous recombination in Drosophila cell lines using CRISPR/Cas9. Biology open. Gratz, S.J., Wildonger, J., Harrison, M.M., and O'Con...Kondo, S., an...

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thetomwilliamslab.com

Useful Links | TheTomWilliamsLab

https://thetomwilliamslab.com/useful-links

The Thomas M. Williams Laboratory at the University of Dayton. Best Gene Inc. Drosophila Injection Services. Bloomington Drosophila Stock Center. San Diego Drosophila Stock Center. CRISPR Resources (O’Connor-Giles Lab). CRISPR Resources (Zhang lab). Developmental Studies Hybridoma Bank. Nucleic Acid Purification and Analysis. OligoCalc: Oligonucleotide Properties Calculator. Protein Molecular Weight Calculator. CHAOS DIALIGN sequence alignment tool. DNA Analysis: DNA sequencing Core Facility. Build a web...

blog-biosyn.com

Web resources for the CRISPR Cas System! – Bio-Synthesis, Inc.

https://blog-biosyn.com/2014/01/15/web-resources-for-the-crispr-cas-system

Custom Peptide Synthesis, Custom Antibody , Custom DNA, Custom RNA ,Custom BNA COMMITED TO BIOMIC RESEARCH since 1984. Web resources for the CRISPR Cas System! Web resources for the CRISPR Cas System! By Klaus D. Linse. January 15, 2014. Bull; ( 1. Http:/ www.oxfcrispr.org. Oxford Fly CRISPR Resources. Http:/ www.crisprflydesign.org/. Offers Cas9 transgenic stocks. Http:/ www.flyrnai.org/crispr/. A web tool to identify CRISPRs for fly study. Http:/ flycrispr.molbio.wisc.edu/. Crass: The CRISPR Assembler.

crisprflydesign.org

Links – CRISPR fly design

http://www.crisprflydesign.org/links

CRISPR fly design Drosophila genome engineering. T7 endo I assay. ECRISPR target finder (Boutros lab). FlyCRISPR target finder (O’Connor-Giles lab). DRSC target finder (Drosophila RNAi Screening Center). Bloomington CRISPR fly stocks. FlyCRISPR Google discussion group. German Cancer Research Center (DKFZ). Division of Signaling and Functional Genomics. Im Neuenheimer Feld 580. F.port@dkfz.de. X6d;.boutros@dkfz.de. Sbullock-at-mrc-lmb.cam.ac.uk. Responsible for website content and design: Fillip Port.

thebestgene.com

BestGene Inc. - CRISPR Info

http://www.thebestgene.com/CRISPRInfoPage.do

Genome Editing Service, pls see here. For currently available Cas9 stocks at BestGene, pls see here. Random indels can be introduced into DSB via NHEJ. Currently, CRISPR/Cas9 has grown in popularity for Drosophila. In the application of CRISPR/Cas9 system in Drosophila. Genome editing, the two RNAs that play key roles can be synthesized into a single chimeric RNA (chiRNA or gRNA). By editing the 20 nt targeting sequence of gRNA, the system introduces DSB on the target sequence followed by an NGG or N...

fgr.hms.harvard.edu

Find CRISPRs | DRSC/TRiP Functional Genomics Resources

http://fgr.hms.harvard.edu/find-crisprs

Skip to main content. DRSC/TRiP Functional Genomics Resources. In vivo fly RNAi (TRiP). In vivo CRISPR (TRiP). Disease-related studies in the fly model. Beta test site: Drosophila Protocols Portal. Shipping reagent library plates. In vivo RNAi fly stocks and vectors. In vivo CRISPR fly stocks and vectors. Drosophila cultured cell lines. Cell CRISPR screen reagents. CRISPR modified cell lines. This tool provides access to a pre-computed database of short guide RNAs (gRNAs) for CRISPR approaches in. 2015 I...

igtrcn.org

CRISPR/Cas Web Resources : IGTRCN

http://igtrcn.org/knowledgebase/crisprcas-web-resources

Insect Genetic Technologies Service Providers. 2015 Technical Workshop – Album. Web Resources for design and planning of CRISPR/Cas9 studies. From Bassett and Liu (2014) CRISPR/Cas9 and Genome Editing in Drosophila. J. Genetics and Genomics 41, 7-19 doi.10.1016/j.gg.2013.12.004. Http:/ www.oxfcrispr.org/. Oxford Fly CRISPR Resources. Bassett et al., 2013. Http:/ www.crisprflydesign.org/. Offers Cas9 transgenic stocks. DRSC CRISPR finder (Perrimon Lab). Http:/ www.flyrnai.org/crispr/. Ren et al., 2013.

unihuaii.com

CRISPR/Cas9系统_联合华益

http://www.unihuaii.com/Recruit/jiyinzubianji

Double-strand break, DSB),. Non-homologous end joining, NHEJ). Homologous recombination, HR). CRISPR/Cas (clustered regularly interspaced palindromic repeats/CRISPR associated,. DRSC Find CRISPR Tool. FlyCRISPR Optimal Target Finder. Dmelanogaster release 5, release 6,. Bassett AR, Tibbit C, Ponting CP, Liu JL (2013) Highly efficient targeted mutagenesis of. With the CRISPR/Cas9 system. Kondo S, Ueda R (2013) Highly improved gene targeting by germline-specific Cas9 expression in. Y[1] M{vas-Cas9.RFP-...

fgr.hms.harvard.edu

CRISPR Efficiency Prediction | DRSC/TRiP Functional Genomics Resources

http://fgr.hms.harvard.edu/crispr-efficiency

Skip to main content. DRSC/TRiP Functional Genomics Resources. In vivo fly RNAi (TRiP). In vivo CRISPR (TRiP). Disease-related studies in the fly model. Beta test site: Drosophila Protocols Portal. Shipping reagent library plates. In vivo RNAi fly stocks and vectors. In vivo CRISPR fly stocks and vectors. Drosophila cultured cell lines. Cell CRISPR screen reagents. CRISPR modified cell lines. Http:/ www.flyrnai.org/evaluateCrispr/. Please see our CRISPR online tools documentation page. 2015 Identificatio...

salehlab.eu

Useful Links - Saleh Lab

http://salehlab.eu/how-we-do-it/useful-links

How We Do It. Our Supporters & Funding. How We Do It. Our Supporters & Funding. Here is a collection of online databases and information that we find useful and relevant to our research. Whether we are looking for genomic tools to make transgenic flies or checking the latest publications on RNAi, these are some of the sites we visit regularly. National Center for Biotechnology Information database (PubMed, GenBank, BLAST. Gene and gene product classification by function. Genes and genomes database. Commo...

rainbowgene.com

Services Rainbow Transgenic Flies, Inc.

http://www.rainbowgene.com/services.html

Of embryo injection/ Standard Service. BAC DNA Injection ( 30kb). BAC DNA Injection ( 30kb). DNA preparation ( 30kb). Balancing lines for P-element based injection. Balancing lines for phic31 based injection. Packing and handling/per order. Discovered in the 1970s, P element. Is a transposon that is present specifically in Drosophila melanogaster and is used widely for mutagenesis and creation of genetically modified flies used for genetic research. P-element injection can. We offer one round of free sta...

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flycrispr.molbio.wisc.edu

Overview | flyCRISPR

Skip to main content. Recognizing the potential of harnessing this system for precise genome engineering in other organisms, Jinek and colleagues identified a minimal two-component system required for the site-specific cleavage of DNA: Cas9 and a chimeric RNA (chiRNA) comprising the crRNA and tracrRNA from S. pyogenes (JINEK et al. 2012). Thus, in this modified CRISPR RNA/Cas9 system a common nuclease is directed to specific DNA sequences by a short, readily generated RNA. As they become available. 2013 ...

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