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NEB offers restriction enzymes, endonucleases, recombinant enzymes, PCR qPCR reagents, expression systems, markers, competent cells and reagents for RNA analysis, polymerases, modifying enzymes, nucleic acids, RNAi and RNA enzymes, cellular imaging, RNAi and RNA enzymes
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DNA Sequences and Maps Tool | New England Biolabs
http://international.neb.com/sitecore/content/nebsg/home/tools-and-resources/interactive-tools/dna-sequences-and-maps-tool
Skip to main content. DNA Sequences and Maps Tool. Customized Solutions and OEM. Molecular Biology Summer Workshops. New England Biolabs Inc. Monday - Friday 9:00 AM - 8:00 PM EST. New England Biolabs Inc. Customized Solutions and OEM. New England Biolabs Inc. New England Biolabs Inc. Monday - Friday 9:00 AM - 5:00 PM EST. Ipswich, MA 01938-2723. International Ordering and Support. Contact Information for US Sales Inquiries and Support. Ready to go Mobile? Download our Android App. Ready to go Mobile?
In vitro digestion of DNA with Cas9 Nuclease, S. pyogenes (M0386) | New England Biolabs
http://international.neb.com/protocols/2014/05/01/in-vitro-digestion-of-dna-with-cas9-nuclease-s-pyogenes-m0386
Skip to main content. Digestion of DNA with Cas9 Nuclease,. Protocols.io also provides an interactive version of this protocol. Where you can discover and share optimizations with the research community. Cas9) is a double-stranded DNA endonuclease that is guided to its target by sequence complementarity of a small RNA loaded into the protein. This protocol describes how to digest double-stranded DNA. Using Cas9 and a single guide RNA (sgRNA). 10X Cas9 Nuclease Reaction Buffer. Reactions are typically 30 ...
Ligation Protocol with T4 DNA Ligase (M0202) | New England Biolabs
http://international.neb.com/protocols/1/01/01/dna-ligation-with-t4-dna-ligase-m0202
Skip to main content. Ligation Protocol with T4 DNA Ligase (M0202). Protocols.io also provides an interactive version of this protocol. Where you can discover and share optimizations with the research community. Set up the following reaction in a microcentrifuge tube on ice. T4 DNA Ligase should be added last. Note that the table shows a ligation using a molar ratio of 1:3 vector to insert for the indicated DNA sizes.). To calculate molar ratios. 20 μl REACTION. T4 DNA Ligase Buffer (10X)*. To 20 μl.
PCR Using Q5® High-Fidelity DNA Polymerase (M0491) | New England Biolabs
http://international.neb.com/protocols/2013/12/13/pcr-using-q5-high-fidelity-dna-polymerase-m0491
Skip to main content. PCR Using Q5 High-Fidelity DNA Polymerase (M0491). Protocols.io also provides an interactive version of this protocol. Where you can discover and share optimizations with the research community. Please note that protocols with Q5 High-Fidelity DNA Polymerase may differ from protocols with other polymerases. Conditions recommended below should be used for optimal performance. 25 µl REACTION. 50 µl REACTION. 10 µM Forward Primer. 10 µM Reverse Primer. Q5 High-Fidelity DNA Polymerase.
PCR Selection Tool | New England Biolabs
http://international.neb.com/sitecore/content/nebsg/home/tools-and-resources/interactive-tools/pcr-selection-tool
Skip to main content. For over 35 years, New England Biolabs, Inc. has been a world leader in the discovery and production of reagents for the life science industry. NEB offers a wide range of DNA polymerases and, through our commitment to research, ensures the development of innovative, high-quality tools for PCR. When choosing a polymerase for PCR, we recommend starting with One. For routine PCR applications, and NEBNext High-Fidelity Master Mix. Indicates recommended choice for application. New Englan...
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Diagnostica Skalpeli
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CST Western Blotting Reagenzien - New England Biolabs
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Klonierung / Synthetische Biologie. PCR / DNA Amplifikation. DNA / RNA Leitern und Marker. Für Informationen zu einzelnen. Produkten besuchen Sie unseren Shop. XP Rabbit Monoclonal Antibodies. PathScan ELISA & Arrays. Proteomics / PTMScan Service & Kits. Für Informationen zu einzelnen. Produkten besuchen Sie unseren Shop. Western Blotting ist eine häufig genutzte Technik zur Detektion von Proteinen in Zell- oder Gewebe-Extrakten. Und die Einhaltung validierter Protokolle. Oder auf cellsignal.com.
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Klonierung / Synthetische Biologie. PCR / DNA Amplifikation. DNA / RNA Leitern und Marker. Für Informationen zu einzelnen. Produkten besuchen Sie unseren Shop. XP Rabbit Monoclonal Antibodies. PathScan ELISA & Arrays. Proteomics / PTMScan Service & Kits. Für Informationen zu einzelnen. Produkten besuchen Sie unseren Shop. 2 Nutzung und Weitergabe personenbezogener Daten. 3 Verwendung von Cookies. NEB verwendet verschiedene Arten von Cookies:. Session Cookies werden nur für die Dauer Ihres Besuchs auf Ihr...
Produktvalidierung bei CST - New England Biolabs GmbH
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Klonierung / Synthetische Biologie. PCR / DNA Amplifikation. DNA / RNA Leitern und Marker. Für Informationen zu einzelnen. Produkten besuchen Sie unseren Shop. XP Rabbit Monoclonal Antibodies. PathScan ELISA & Arrays. Proteomics / PTMScan Service & Kits. Für Informationen zu einzelnen. Produkten besuchen Sie unseren Shop. Antikörper-Validierung Ein wichtiges und aktuelles Thema in der wissenschaftlichen Gemeinschaft and bei CST. Workshop Antibody Validation: Standards, Policies, and Practices. Cell Signa...
CST XP Rabbit Monoclonal Antibodies - NEB GmbH
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Klonierung / Synthetische Biologie. PCR / DNA Amplifikation. DNA / RNA Leitern und Marker. Für Informationen zu einzelnen. Produkten besuchen Sie unseren Shop. XP Rabbit Monoclonal Antibodies. PathScan ELISA & Arrays. Proteomics / PTMScan Service & Kits. Für Informationen zu einzelnen. Produkten besuchen Sie unseren Shop. XP Rabbit Monoclonal Antibodies. Die besten Kaninchen Monoclonals:. Rabbit Monoclonal Antibodies – eXceptional Performance garantiert! Nur die besten Antikörper erhalten das Prädikat XP.
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Klonierung / Synthetische Biologie. PCR / DNA Amplifikation. DNA / RNA Leitern und Marker. Für Informationen zu einzelnen. Produkten besuchen Sie unseren Shop. XP Rabbit Monoclonal Antibodies. PathScan ELISA & Arrays. Proteomics / PTMScan Service & Kits. Für Informationen zu einzelnen. Produkten besuchen Sie unseren Shop. Außer im Internetshop können Sie Bestellungen per e-mail, Fax, Telefon oder Post aufgeben. Senden Sie Ihre Bestellungen bitte an:. New England Biolabs GmbH. 65926 Frankfurt am Main.
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Klonierung / Synthetische Biologie. PCR / DNA Amplifikation. DNA / RNA Leitern und Marker. Für Informationen zu einzelnen. Produkten besuchen Sie unseren Shop. XP Rabbit Monoclonal Antibodies. PathScan ELISA & Arrays. Proteomics / PTMScan Service & Kits. Für Informationen zu einzelnen. Produkten besuchen Sie unseren Shop. New England Biolabs GmbH. Brüningstr. 50; Geb. B852. D-65926 Frankfurt am Main. Vertreten durch den Geschäftsführer:. Mo – Fr. von 08:30 – 17:00 Uhr. DE 113 867 607. DRES DE FF 566.
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Klonierung / Synthetische Biologie. PCR / DNA Amplifikation. DNA / RNA Leitern und Marker. Für Informationen zu einzelnen. Produkten besuchen Sie unseren Shop. XP Rabbit Monoclonal Antibodies. PathScan ELISA & Arrays. Proteomics / PTMScan Service & Kits. Für Informationen zu einzelnen. Produkten besuchen Sie unseren Shop. 0800/246-5227 (kostenfrei für Kunden aus Deutschland). 00800/246-52277 (kostenfrei für Kunden aus Österreich). New England Biolabs GmbH. Brüningstr. 50; Geb. B852.
Flow-validierte Antikörper von CST - New England Biolabs
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Klonierung / Synthetische Biologie. PCR / DNA Amplifikation. DNA / RNA Leitern und Marker. Für Informationen zu einzelnen. Produkten besuchen Sie unseren Shop. XP Rabbit Monoclonal Antibodies. PathScan ELISA & Arrays. Proteomics / PTMScan Service & Kits. Für Informationen zu einzelnen. Produkten besuchen Sie unseren Shop. Von Cell Signaling Technology erfüllen alle Kriterien für einen erfolgreichen und reproduzierbaren Einsatz in der Durchflusszytometrie! Kommen zu Ihnen mit Geld-zurück-Garantie. Brüning...
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Skip to main content. Cas9 Nuclease NLS,. Cas9 Nuclease NLS,. Like other filarial nematode parasites, canine heartworm plays host to the Wolbachia endosymbiont. Transcriptomic studies over the life cycles of both organisms shed light on vulnerabilities for therapeutic targeting. What is ligation bias, and how does it impact high-throughput Small RNA sequencing? This article explores the origins of ligation bias, and makes recommendations for adaptor design to minimize bias in HTS. 3 -terminal phosphate o...
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