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JCat: A novel tool to adapt codon usage of a target gene to its potential expression host.

Bioinformatic Tool to adapt Codon Usage to sequenced prokaryotes.

http://www.jcat.de/

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JCat: A novel tool to adapt codon usage of a target gene to its potential expression host. | jcat.de Reviews
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Bioinformatic Tool to adapt Codon Usage to sequenced prokaryotes.
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caicaculation,introduction,screenshots,literature,links,download,nucleic acids research,codon adaptation,to change,dna/rna sequence,protein sequence,3 select organism,arabidopsis thaliana,caenorhabditis elegans,drosophila melanogaster,homo sapiens,aatii
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JCat: A novel tool to adapt codon usage of a target gene to its potential expression host. | jcat.de Reviews

https://jcat.de

Bioinformatic Tool to adapt Codon Usage to sequenced prokaryotes.

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1

JCat: A novel tool to adapt codon usage of a target gene to its potential expression host.

http://www.jcat.de/Links.jsp

Bioinformatic tools from our team:. JCat was published in NAR. PRODORIC - Prokaryotic Database of Gene Regulation and Regulatory Networks. JVirGel - Virtual 2D-protein gels. PrediSi - PREDIction of SIgnal peptides. REBASE - The Restriction Enzyme Database. TransTerm - Transcription Terminators in bacterial genomes. 2004-2008 by Andreas Grote • andreas.grote@tu-bs.de.

2

JCat: A novel tool to adapt codon usage of a target gene to its potential expression host.

http://www.jcat.de/ChangeTranslation.jsp

Bioinformatic tools from our team:. JCat was published in NAR. Change applied code for translation. Genetic code used for the input sequence (Default: Standard genetic code):. Translation Input Sequence - - - - - - - - - - - -. Mold Mitochondrial; Protozoan Mitochondrial; Coele. Ciliate Nuclear; Dasycladacean Nuclear; Hexamita N. Echinoderm Mitochondrial; Flatworm Mitochondrial. Bacterial and Plant Plastid. 2004-2008 by Andreas Grote • andreas.grote@tu-bs.de.

3

JCat: A novel tool to adapt codon usage of a target gene to its potential expression host.

http://www.jcat.de/Introduction.jsp

Bioinformatic tools from our team:. JCat was published in NAR. The CodonAdaptationTool (JCAT) presents a simple method to adapt the Codon Usage to most sequenced prokaryotic organisms and selected eukaryotic organisms. The codon adaptation plays a major role in cases where foreign genes are expressed in hosts and the codon usage of the host differs from that of the organism where the gene stems from. Unadapted codons in the host can for example lead to a minor expression rate.

4

JCat: A novel tool to adapt codon usage of a target gene to its potential expression host.

http://www.jcat.de/Screenshots.jsp

Bioinformatic tools from our team:. JCat was published in NAR. In this example the CodonUsage of the gene 'anr' from Pseudomonas aeruginosa. Was adapted to Bacillus subtilis. The first screenshot shows the Codon Usage in B. subtilis. Before the codon Adaptation:. The red line shows the Codon Usage for each codon present in the gene. The blue line depicts the mean Codon Usage in B. subtilis. The second screenshot shows the adapted Codon Usage for this gene in B. subtilis.

5

JCat: A novel tool to adapt codon usage of a target gene to its potential expression host.

http://www.jcat.de/Download.jsp

Bioinformatic tools from our team:. JCat was published in NAR. It is strongly recommended to use the jar-file. This version of JCat is the most advanced and runs under all sytems. Updated jar-File works now under all systems! This version requires Java 1.5 or higher. Instructions for the jar-File:. 1 Open shell or terminal. 2 Type: "java -jar jcat.jar". Application: jcat.tar.gz. Install instructions for the Linux-application:. 1 Unpack the package. Install instructions for the Windows-application:.

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prodoric.tu-bs.de prodoric.tu-bs.de

PRODORIC Release 8.9

http://prodoric.tu-bs.de/index.php?index=2

Query Database - Brief Introduction. Step 1 - Select Query Form. Select a query form from the menu or click on the image. The following forms and information contents are provided:. Gene and operon structure, genomic context, codon usage, GC content. Amino acid sequence, structural and functional information, interactions with other proteins and DNA binding sites. Information about DNA bindings sites of transcriptional regulators, sequence, genomic context, mode of interaction.

prodoric.de prodoric.de

PRODORIC Release 8.9

http://www.prodoric.de/index.php?index=2

Query Database - Brief Introduction. Step 1 - Select Query Form. Select a query form from the menu or click on the image. The following forms and information contents are provided:. Gene and operon structure, genomic context, codon usage, GC content. Amino acid sequence, structural and functional information, interactions with other proteins and DNA binding sites. Information about DNA bindings sites of transcriptional regulators, sequence, genomic context, mode of interaction.

prodoric.net prodoric.net

PRODORIC Release 8.9

http://www.prodoric.net/statistics.php

Number of annotated publications:. Number of available genomes:. Aeropyrum pernix (strain K1). Agrobacterium tumefaciens (strain C58 / ATCC 33970 / ATCC 33970 [Dupont]). Bacillus subtilis (strain 168). Bradyrhizobium japonicum (strain USDA 110). Corynebacterium glutamicum (strain DSM 20300 / ATCC 13032 / NCIB 10025 [Kalinowski]). Escherichia coli O6 (strain UPEC / O6:H1 / ATCC 700928 / CFT073). Escherichia coli (strain K12). Escherichia coli (strain Sakai / O157:H7 / RIMD 0509952 / EHEC). Yersinia pseudo...

prodoric.tu-bs.de prodoric.tu-bs.de

PRODORIC Release 8.9

http://prodoric.tu-bs.de/index.php?index=1

Welcome to the PRODORIC. Is a comprehensive database about gene regulation and gene expression in prokaryotes. It includes a manually curated and unique collection of transcription factor binding sites. A variety of bioinformatics tools for the prediction, analysis and visualization of regulons and gene reglulatory networks is included. Click here to query the database. Please cite PRODORIC and results obtained by the use of the database as:. PRODORIC: prokaryotic database of gene regulation.

prodoric.tu-bs.de prodoric.tu-bs.de

PRODORIC Release 8.9

http://prodoric.tu-bs.de/statistics.php

Number of annotated publications:. Number of available genomes:. Aeropyrum pernix (strain K1). Agrobacterium tumefaciens (strain C58 / ATCC 33970 / ATCC 33970 [Dupont]). Bacillus subtilis (strain 168). Bradyrhizobium japonicum (strain USDA 110). Corynebacterium glutamicum (strain DSM 20300 / ATCC 13032 / NCIB 10025 [Kalinowski]). Escherichia coli O6 (strain UPEC / O6:H1 / ATCC 700928 / CFT073). Escherichia coli (strain K12). Escherichia coli (strain Sakai / O157:H7 / RIMD 0509952 / EHEC). Yersinia pseudo...

prodoric.de prodoric.de

PRODORIC Release 8.9

http://www.prodoric.de/index.php?index=1

Welcome to the PRODORIC. Is a comprehensive database about gene regulation and gene expression in prokaryotes. It includes a manually curated and unique collection of transcription factor binding sites. A variety of bioinformatics tools for the prediction, analysis and visualization of regulons and gene reglulatory networks is included. Click here to query the database. Please cite PRODORIC and results obtained by the use of the database as:. PRODORIC: prokaryotic database of gene regulation.

prodoric.net prodoric.net

PRODORIC Release 8.9

http://www.prodoric.net/index.php?index=1

Welcome to the PRODORIC. Is a comprehensive database about gene regulation and gene expression in prokaryotes. It includes a manually curated and unique collection of transcription factor binding sites. A variety of bioinformatics tools for the prediction, analysis and visualization of regulons and gene reglulatory networks is included. Click here to query the database. Please cite PRODORIC and results obtained by the use of the database as:. PRODORIC: prokaryotic database of gene regulation.

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JCat: A novel tool to adapt codon usage of a target gene to its potential expression host.

Bioinformatic tools from our team:. JCat was published in NAR. 1 Type/paste sequences below:. Standard genetic code is used for the input sequence. Click here. 2 Specify the pasted Sequence:. Eukaryotes - - - - - - - - - - - -. Aspergillus niger ATCC 1015. Aspergillus niger NRRL 3. Prokaryotes - - - - - - - - - - - -. Acidobacteria bacterium (strain Ellin345). Acinetobacter sp. (strain ADP1). Aeropyrum pernix (strain K1). Agrobacterium tumefaciens (strain C58 / ATCC 33970 / ATCC 33970 [Cereon]). Bacillus...

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