karthikeyan-protocols.blogspot.com

Protocols

Saturday, May 9, 2009. Agarose gels are used to separate different size strands of DNA, consisting of genomic DNA and plasmids, or the products of restriction enzyme. Digests and PCR. The following is a simple step-by-step procedure for running a gel and visualizing DNA fragments by ethidium bromide staining. Prepare the molten gel. Mix the powder agarose with electrophoresis buffer ( TAE. Prepare the gel apparatus. Generally gels are run at approximately 80 - 100 mAmp. A setting any higher will caus...

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Saturday, May 9, 2009. Agarose gels are used to separate different size strands of DNA, consisting of genomic DNA and plasmids, or the products of restriction enzyme. Digests and PCR. The following is a simple step-by-step procedure for running a gel and visualizing DNA fragments by ethidium bromide staining. Prepare the molten gel. Mix the powder agarose with electrophoresis buffer ( TAE. Prepare the gel apparatus. Generally gels are run at approximately 80 - 100 mAmp. A setting any higher will caus...

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1 protocols

2 agarose gel electrophoresis

3 time required

4 approximately 2 hours

5 or tbe

6 pour the gel

7 prepare samples

8 load the gel

9 run the gel

10 stain the gel

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Protocols | karthikeyan-protocols.blogspot.com Reviews

https://karthikeyan-protocols.blogspot.com

Saturday, May 9, 2009. Agarose gels are used to separate different size strands of DNA, consisting of genomic DNA and plasmids, or the products of restriction enzyme. Digests and PCR. The following is a simple step-by-step procedure for running a gel and visualizing DNA fragments by ethidium bromide staining. Prepare the molten gel. Mix the powder agarose with electrophoresis buffer ( TAE. Prepare the gel apparatus. Generally gels are run at approximately 80 - 100 mAmp. A setting any higher will caus...

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karthikeyan-protocols.blogspot.com

1

Protocols: May 2009

http://karthikeyan-protocols.blogspot.com/2009_05_01_archive.html

Saturday, May 9, 2009. Agarose gels are used to separate different size strands of DNA, consisting of genomic DNA and plasmids, or the products of restriction enzyme. Digests and PCR. The following is a simple step-by-step procedure for running a gel and visualizing DNA fragments by ethidium bromide staining. Prepare the molten gel. Mix the powder agarose with electrophoresis buffer ( TAE. Prepare the gel apparatus. Generally gels are run at approximately 80 - 100 mAmp. A setting any higher will caus...

2

Protocols: Agarose Gel Electrophoresis

http://karthikeyan-protocols.blogspot.com/2009/05/agarose-gel-electrophoresis.html

Saturday, May 9, 2009. Agarose gels are used to separate different size strands of DNA, consisting of genomic DNA and plasmids, or the products of restriction enzyme. Digests and PCR. The following is a simple step-by-step procedure for running a gel and visualizing DNA fragments by ethidium bromide staining. Prepare the molten gel. Mix the powder agarose with electrophoresis buffer ( TAE. Prepare the gel apparatus. Generally gels are run at approximately 80 - 100 mAmp. A setting any higher will caus...

3

Protocols: How To Make a Phosphate Buffer

http://karthikeyan-protocols.blogspot.com/2009/05/how-to-make-phosphate-buffer.html

Saturday, May 9, 2009. How To Make a Phosphate Buffer. Because it contains three acidic protons, phosphoric acid has multiple dissociation constants and can be used to create buffers for either of the three corresponding pHs. The three pKa values for phosphoric acid are 2.15, 6.86 and 12.32. Monosodium phosphate and its conjugate base, disodium phosphate are usually used to generate buffers of pH values around 7, for biological applications, as shown here. Time Required: 10 minutes. 4 Solve for [Acid].

4

Protocols: How To Make TAE Buffer

http://karthikeyan-protocols.blogspot.com/2009/05/how-to-make-tae-buffer.html

Saturday, May 9, 2009. How To Make TAE Buffer. Tris-acetate-EDTA (TAE) buffer is historically the most common buffer used for agarose gel electrophoresis in the analyses of DNA products resulting from PCR amplification, DNA purification protocols, or DNA cloning experiments. This buffer has a low ionic strength and low buffering capacity. It is best suited to electrophoresis of large ( 20 kb) pieces of DNA and will need to be replaced frequently or recirculated for longer ( 4 h) gel run times. Subscribe ...

5

Protocols: SDS-PAGE

http://karthikeyan-protocols.blogspot.com/2009/05/sds-page.html

Saturday, May 9, 2009. Sodium dodecyl sulphate polyacrylaminde gel electrophoresis (SDS–PAGE) was performed to separate and observe the protein pattern of the sample by the method of Laemlli (1970). Preparation of stock solution and buffers. A Acrylamide : 29.2 g. B N,N’- methylene-bis-acrylamide : 0.8 g. Add water, dissolve and make upto 100ml and filter with Whatmann No.1 filter paper. 2 Separating gel buffer. A Tris–HCl : 1.5 M, pH 8.8. 3 Stacking gel buffer. A Tris–HCl : 1 M, pH 6.8. C SDS : 0.1%.

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Protocols

Saturday, May 9, 2009. Agarose gels are used to separate different size strands of DNA, consisting of genomic DNA and plasmids, or the products of restriction enzyme. Digests and PCR. The following is a simple step-by-step procedure for running a gel and visualizing DNA fragments by ethidium bromide staining. Prepare the molten gel. Mix the powder agarose with electrophoresis buffer ( TAE. Prepare the gel apparatus. Generally gels are run at approximately 80 - 100 mAmp. A setting any higher will caus...

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