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Desperate Lab Monkeys

Saturday, November 11, 2006. Week 19 - DNA Extraction (contd) - daphne. DNA Extraction ( from the lower layer). 1 remove aqueous phase (containing RNA) COMPLETELY and transfer the organic phase to a clean microcentrifugr tube (remove tissue debri). 2 add 0.3 ml of 100% Ethanol and mix by inversion. 3 incubate at room temperature for 3 min, then centrifuge at 2000. For 5 min at 4 degrees C. 4 remove the phenol-ethanol supernatant and wash the DNA pellet with 1ml of 0.1M of sodium citrate in 10% ethanol.

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Desperate Lab Monkeys | lab-monkeys.blogspot.com Reviews
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Saturday, November 11, 2006. Week 19 - DNA Extraction (contd) - daphne. DNA Extraction ( from the lower layer). 1 remove aqueous phase (containing RNA) COMPLETELY and transfer the organic phase to a clean microcentrifugr tube (remove tissue debri). 2 add 0.3 ml of 100% Ethanol and mix by inversion. 3 incubate at room temperature for 3 min, then centrifuge at 2000. For 5 min at 4 degrees C. 4 remove the phenol-ethanol supernatant and wash the DNA pellet with 1ml of 0.1M of sodium citrate in 10% ethanol.
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1 desperate lab monkeys
2 0 comments
3 internal quality assurance
4 winnie
5 batch of
6 analyses be accepted
7 or rejected
8 1 direct sequencing
9 heyy khadijah
10 are they comparable
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desperate lab monkeys,0 comments,internal quality assurance,winnie,batch of,analyses be accepted,or rejected,1 direct sequencing,heyy khadijah,are they comparable,1 test ordering,2 specimen collection,a postural effects,b collection tubes,d avoiding clots
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Desperate Lab Monkeys | lab-monkeys.blogspot.com Reviews

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Saturday, November 11, 2006. Week 19 - DNA Extraction (contd) - daphne. DNA Extraction ( from the lower layer). 1 remove aqueous phase (containing RNA) COMPLETELY and transfer the organic phase to a clean microcentrifugr tube (remove tissue debri). 2 add 0.3 ml of 100% Ethanol and mix by inversion. 3 incubate at room temperature for 3 min, then centrifuge at 2000. For 5 min at 4 degrees C. 4 remove the phenol-ethanol supernatant and wash the DNA pellet with 1ml of 0.1M of sodium citrate in 10% ethanol.

INTERNAL PAGES

lab-monkeys.blogspot.com lab-monkeys.blogspot.com
1

Desperate Lab Monkeys: July 2006

http://lab-monkeys.blogspot.com/2006_07_01_archive.html

Sunday, July 30, 2006. 5th week - "REAL-TIME" PCR - daphne. Used to quantify gene expression. Two different methods 1. Absolute Quantification. Determines input copy number of the transcript of interest, by relating the PCR signal to a standard curve. Describes the change in expression of the target gene relative to some reference group such as untreated control or sample at time zero in time course study. Data analysis is then done using 2 to the power of delta delta Ct, which is automatically calculate...

2

Desperate Lab Monkeys: week 13- basic tissue culture techniques (daphne)

http://lab-monkeys.blogspot.com/2006/09/week-13-basic-tissue-culture.html

Monday, September 25, 2006. Week 13- basic tissue culture techniques (daphne). This week i learnt some basic tissue culturing techniques. 1) prior to carrying out any experiment, it is important to alwasy wear Personal Protective Equipment (eg lab coat, gloves, goggles). 2) in tissue culture, it is important to swipe the fume hood with ethanol first before starting any experiment. 3) spray gloves and all materials to be used with ethanol first before bringing them into the fume hood. 12) check under the ...

3

Desperate Lab Monkeys: Good Laboratory Practice As A General Analytical Approach

http://lab-monkeys.blogspot.com/2006/09/good-laboratory-practice-as-general.html

Saturday, September 16, 2006. Good Laboratory Practice As A General Analytical Approach. Good Laboratory Practice (GLP). Covers the proper milieu in which the entire investigation including specimen collecting and reporting takes place. These conditions are just as important as the technical quality of an analytical method. The following points within good laboratory pratice are of particular importance:. Manning of the laboratory and eduaction of staff. Organization of the laboratory. They were designed...

4

Desperate Lab Monkeys: reply to ms chew (daphne)

http://lab-monkeys.blogspot.com/2006/09/reply-to-ms-chew-daphne.html

Sunday, September 24, 2006. Reply to ms chew (daphne). Heyy Ms Chew,. Sorry for the late reply regarding your question to my post in week 10: "But what about the quantity of the DNA extraction using both techniques? Thanks you ms chew. Posted by w-w-a-d @ 11:01 PM. Willie Winnie Alvin Daphne. View my complete profile. Pre- analytical factors affecting test results. Good Laboratory Practice As A General Analytical A. Week 10 - DAPHNE- Gel Extraction and Column purifi. Reply to Ms Chew. 7th and 8th Week.

5

Desperate Lab Monkeys: November 2006

http://lab-monkeys.blogspot.com/2006_11_01_archive.html

Saturday, November 11, 2006. Week 19 - DNA Extraction (contd) - daphne. DNA Extraction ( from the lower layer). 1 remove aqueous phase (containing RNA) COMPLETELY and transfer the organic phase to a clean microcentrifugr tube (remove tissue debri). 2 add 0.3 ml of 100% Ethanol and mix by inversion. 3 incubate at room temperature for 3 min, then centrifuge at 2000. For 5 min at 4 degrees C. 4 remove the phenol-ethanol supernatant and wash the DNA pellet with 1ml of 0.1M of sodium citrate in 10% ethanol.

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...Guinea (^00^) @ work...: October 2006

http://guineab4u.blogspot.com/2006_10_01_archive.html

Guinea ( 00 ) @ work. An anchor point where we communicate visually, share our self-experiences, learn from each others' entries and accept feedback from our fellow peers. Guinea ( 00 ) leader: Devi.Guinea ( 00 ) trainee: Kailing, SiangFang and JingShya. Saturday, October 28, 2006. Different Kinds of Blood Tubes. Fluoride (Glucose) Tubes (Gray). SST Tubes (serum separator tubes) (Gold). PST Tubes (plasma separator tubes) (Green). Acid Citrate Dextrose (ACD). Sedimentation Rate Determination (SRD). Contai...

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...Guinea (^00^) @ work...: August 2006

http://guineab4u.blogspot.com/2006_08_01_archive.html

Guinea ( 00 ) @ work. An anchor point where we communicate visually, share our self-experiences, learn from each others' entries and accept feedback from our fellow peers. Guinea ( 00 ) leader: Devi.Guinea ( 00 ) trainee: Kailing, SiangFang and JingShya. Tuesday, August 29, 2006. Posted by Guinea ( 00 ) @ 11:37 PM. Sunday, August 27, 2006. Reply to Pei Qing's Questions. Posted by: Siang Fang. What is Fanconi Anaemia? Physical Characteristics of Individuals with Fanconi Anemia. Discoloration of the skin.

guineab4u.blogspot.com guineab4u.blogspot.com

...Guinea (^00^) @ work...: September 2006

http://guineab4u.blogspot.com/2006_09_01_archive.html

Guinea ( 00 ) @ work. An anchor point where we communicate visually, share our self-experiences, learn from each others' entries and accept feedback from our fellow peers. Guinea ( 00 ) leader: Devi.Guinea ( 00 ) trainee: Kailing, SiangFang and JingShya. Sunday, September 24, 2006. Hema-Tek 2000 Stainer and Storage of Slides/Specimens. Posted by: Siang Fang. Start-up of Hema-Tek 2000 Stainer. Remove the cover from the front panel. Switch on the stainer. During Operation of Hema-Tek 2000 Stainer. If the s...

guineab4u.blogspot.com guineab4u.blogspot.com

...Guinea (^00^) @ work...: July 2006

http://guineab4u.blogspot.com/2006_07_01_archive.html

Guinea ( 00 ) @ work. An anchor point where we communicate visually, share our self-experiences, learn from each others' entries and accept feedback from our fellow peers. Guinea ( 00 ) leader: Devi.Guinea ( 00 ) trainee: Kailing, SiangFang and JingShya. Sunday, July 30, 2006. Posted by Guinea ( 00 ) @ 8:34 PM. Saturday, July 29, 2006. Aseptic Technique in Cytogenetic Laboratory. Posted by: Siang Fang. Cytogenetics is the study of the structure of chromosome material. It includes routine analysis of ...

simplylmqa.blogspot.com simplylmqa.blogspot.com

BLOGGERS: jane, maybelline, valerie, huiyan: June 2006

http://simplylmqa.blogspot.com/2006_06_01_archive.html

BLOGGERS: jane, maybelline, valerie, huiyan. Thursday, June 29, 2006. First week of work. When I first stepped into the workplace, there are signs and labels that catches my eye. Especially the radioactive sign label at my workplace. Inside the lab, there are a few workbench areas (named "Dangerous" or Danger area are labelled with orange-coloured tape) allocated for dealing with some chemicals such as EtBr (Ethidium bromide) and carcinogens. The dangerous area is considered as contaminated. The workbenc...

medtechinprog.blogspot.com medtechinprog.blogspot.com

*~Student Internship Program~*

http://medtechinprog.blogspot.com/2006/10/calibration-of-pipettes-pei-qing.html

Student Internship Program *. Tuesday, October 10, 2006. Note: This was supposed to be posted on September 8 - Week 11. This week I experienced what Ms Chew mentioned before last time in class. Calibrate pipettes in a room for one whole day. Performance Check on Piston Operated Volumetric Apparatus (POVA). Or what we call: Pipettes. The method used to assess the accuracy and precision of the pipettes is the gravimetric method. National Committee for Clinical Laboratory Standards or NCCLS. 2 Collect Type ...

slaveryinpoly.blogspot.com slaveryinpoly.blogspot.com

Experiencing [S]lavery [I]n [P]oly: July 2006

http://slaveryinpoly.blogspot.com/2006_07_01_archive.html

Experiencing [S]lavery [I]n [P]oly. Letting you guys have a glimpse of how four interns surviving 5 months of [S][I][P]. Slaves involved: Head Slave -Khadijah Loyal Slaves -Christopher -Shikin -Fadilah. Sunday, July 30, 2006. Apart from urine and stool samples, the routine section also handles other body fluids like the synovial fluid which is found in the joints especially the knees. These samples are first spinned down to concentrate it before a drop of it is placed onto a microscopic slide and sta...

slaveryinpoly.blogspot.com slaveryinpoly.blogspot.com

Experiencing [S]lavery [I]n [P]oly: August 2006

http://slaveryinpoly.blogspot.com/2006_08_01_archive.html

Experiencing [S]lavery [I]n [P]oly. Letting you guys have a glimpse of how four interns surviving 5 months of [S][I][P]. Slaves involved: Head Slave -Khadijah Loyal Slaves -Christopher -Shikin -Fadilah. Monday, August 21, 2006. Hey people of the world! Ok for this week, im going to try to blog all that ive learnt while i was attached to the HIV section of the current private company that im attached to. Frankly speaking, i initially thought that in the HIV section u only run HIV test but boy! Is an intra...

hakk00.blogspot.com hakk00.blogspot.com

SIP Experience: October 2006

http://hakk00.blogspot.com/2006_10_01_archive.html

Wednesday, October 25, 2006. My lab participates in Biorad Unity interlaboratory program. This interlaboratory program is performed by Biorad. It is done every month where the control values that are run everyday are sent to Biorad where they will compare your lab values with all the other lab participating in that particular program. This program has a lot of advantages and is one of the most powerful tool at the moment. Be one of thousands of lab site participants worldwide, more than any other. Withou...

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Desperate Lab Monkeys

Saturday, November 11, 2006. Week 19 - DNA Extraction (contd) - daphne. DNA Extraction ( from the lower layer). 1 remove aqueous phase (containing RNA) COMPLETELY and transfer the organic phase to a clean microcentrifugr tube (remove tissue debri). 2 add 0.3 ml of 100% Ethanol and mix by inversion. 3 incubate at room temperature for 3 min, then centrifuge at 2000. For 5 min at 4 degrees C. 4 remove the phenol-ethanol supernatant and wash the DNA pellet with 1ml of 0.1M of sodium citrate in 10% ethanol.

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