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Lab Scribbles

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Lab Scribbles | labscribbles.com Reviews
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Lab Scribbles | labscribbles.com Reviews

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Purifying full-length huntingtin protein from baculovirus expression system for EM analysis – Lab Scribbles

http://labscribbles.com/2016/09/21/purifying-full-length-huntingtin

Real-time open access science. Purifying full-length huntingtin protein from baculovirus expression system for EM analysis. Purifying full-length huntingtin protein from baculovirus expression system for EM analysis. September 21, 2016. September 21, 2016. Things are moving fast in the lab which is exciting as it means the project is moving forward quickly too. However, it does mean that I now have lots of data to share in this post! Thanks again Ihn Sik! 8211; the protein was very aggregated meaning tha...

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January 2017 – Lab Scribbles

http://labscribbles.com/2017/01

Real-time open access science. Validating huntingtin domain protein samples. January 31, 2017. Last week I did some experiments to check that the protein samples I made 1) are what I think they are i.e. not a contaminant in the sample and 2) are good quality protein – all data on Zenodo. The protein is definitely the right amino acid sequence and it is not contaminated – wahey! However, Read More …. Making huntingtin domains and exciting EM updates. January 23, 2017. January 23, 2017. January 10, 2017.

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Bio – Lab Scribbles

http://labscribbles.com/bio

Real-time open access science. My name is Dr. Rachel Harding. I studied at Oxford University for both my undergraduate degree, Molecular and Cellular Biochemistry, as well as my graduate degree, DPhil Pathology. During my DPhil in the lab of Professor Susan Lea. I learnt much about recombinant protein expression, purification, biophysical methods and structural biology under her supervision. University of Toronto, working for Dr Cheryl Arrowsmith. Currently, I am doing research into the structure of hunt...

4

More progress on huntingtin cryo-EM – Lab Scribbles

http://labscribbles.com/2016/12/19/more-progress-on-huntingtin-cryo-em

Real-time open access science. More progress on huntingtin cryo-EM. More progress on huntingtin cryo-EM. December 19, 2016. I was over the moon last week to receive an update (available here). I have also been doing some more protein purification of both the full-length huntingtin protein. As well as shorter constructs. I am wrapping up for the holidays in the lab this week but will be posting any updates here soon! Click to share on Twitter (Opens in new window). Leave a Reply Cancel reply. On Domain ma...

5

General – Lab Scribbles

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Real-time open access science. Optimisation of huntingtin domain protein sample buffer conditions. February 1, 2017. Previously, I wrote about how I successfully made a small chunk of the huntingtin protein. However, I then found that the sample was not very thermally stable. Therefore I have tried to improve the protein stability by looking at different solution conditions for the protein molecules by screening different buffers, pH, salt concentrations as well Read More …. January 31, 2017. 2017 is wel...

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Some Notes from OpenCon 2016 Toronto | Vibrant Outlook

https://vibrantoutlook.wordpress.com/2016/11/28/some-notes-from-opencon-2016-toronto

Exploring educational technologies and digital storytelling. Some Notes from OpenCon 2016 Toronto. This past Saturday a group of enthusiastic Open Access advocates met to attend OpenCon 2016 Toronto held at TWG (The Working Group) on Adelaide St. in Toronto. Lorraine Chuen, Co-Founder of OOO Canada Research Network hosted the event. Open Access and Social Justice: Aligning Open Access with the Mission of the Public University. Contributions to Science Research. David Porter, the new CEO of eCampus Ontario.

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OpenCon 2016 – Megan Beckett

https://meganbeckett.wordpress.com/2016/11/24/opencon-2016

Exploring, learning, reflecting. November 24, 2016. Last week I got back from the US after attending OpenCon. 8220;OpenCon is more than a conference. It’s a platform for the next generation to learn about Open Access, Open Education, and Open Data, develop critical skills, and catalyze action toward a more open system for sharing the world’s information from scholarly and scientific research, to educational materials, to digital research data.”. Hosted around the world throughout the year. A large propor...

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SGC | News from SGC

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Skip to main content. A public-private partnership that supports the discovery of new medicines through open access research. Strategic Alliances and Communications. TARGET ENABLING PACKAGES (TEPs). Probes against other protein classes. Structure-guided Drug Discovery Coalition (SDDC). Faculty and Industry Participants. Structural and Chemical Biology Bootcamp. High Throughput Protein Crystallisation. SGC Director Aled Edwards. Peter J. Brown. Alex N. Bullock. Katlin B. Massirer. Mario H. Bengtson. For a...

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Sally Wilson | Vibrant Outlook

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Exploring educational technologies and digital storytelling. Author Archives: Sally Wilson. Some Notes from OpenCon 2016 Toronto. This past Saturday a group of enthusiastic Open Access advocates met to attend OpenCon 2016 Toronto held at TWG (The Working Group) on Adelaide St. in Toronto. Lorraine Chuen, Co-Founder of OOO Canada Research Network hosted the event. Open Access and Social Justice: Aligning Open Access with the Mission of the Public University. Contributions to Science Research. Beyond the e...

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SGC | News from SGC

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Skip to main content. A public-private partnership that supports the discovery of new medicines through open access research. Strategic Alliances and Communications. TARGET ENABLING PACKAGES (TEPs). Probes against other protein classes. Structure-guided Drug Discovery Coalition (SDDC). Faculty and Industry Participants. Structural and Chemical Biology Bootcamp. High Throughput Protein Crystallisation. SGC Director Aled Edwards. Peter J. Brown. Alex N. Bullock. Katlin B. Massirer. Mario H. Bengtson. For a...

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Lab Scribbles

Real-time open access science. January 10, 2017. 2017 is well and truly underway and progress is ongoing for the huntingtin structure project. Prof Susan Lea has been busy collecting data for me on her microscope throughout the holidays. This approach, called cry-electron microscopy, which allows us to image individual particles of the huntingtin molecule. Susan has worked hard to Read More …. More progress on huntingtin cryo-EM. December 19, 2016. December 5, 2016. Back in the lab! November 29, 2016.

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