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From Micrographs to Particle Stack (Preprocessing). Particle Coordinate File formats. Defocus Determination Using Samdefocus.py. 2D classification and particle screening. FREALIGN Using Samfrealign.py. Preparation of Negative Stain Solution and EM Grids. SPIDER 3D Projection Matching Using Samrefine.py. Simple Steps for T12 Scope. We use high-resolution cryo-electron microscopy (cryo-EM) to study the structure and function of membrane proteins and DNA-protein machines. We moved to SGM-509!

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Home | Liao Lab | liao.hms.harvard.edu Reviews

https://liao.hms.harvard.edu

From Micrographs to Particle Stack (Preprocessing). Particle Coordinate File formats. Defocus Determination Using Samdefocus.py. 2D classification and particle screening. FREALIGN Using Samfrealign.py. Preparation of Negative Stain Solution and EM Grids. SPIDER 3D Projection Matching Using Samrefine.py. Simple Steps for T12 Scope. We use high-resolution cryo-electron microscopy (cryo-EM) to study the structure and function of membrane proteins and DNA-protein machines. We moved to SGM-509!

INTERNAL PAGES

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1

People | Liao Lab

http://liao.hms.harvard.edu/people

From Micrographs to Particle Stack (Preprocessing). Particle Coordinate File formats. Defocus Determination Using Samdefocus.py. 2D classification and particle screening. FREALIGN Using Samfrealign.py. Preparation of Negative Stain Solution and EM Grids. SPIDER 3D Projection Matching Using Samrefine.py. Simple Steps for T12 Scope. Maofu liao@hms.harvard.edu. Melissa chambers@hms.harvard.edu. Min Luo@hms.harvard.edu. Wei mi@hms.harvard.edu. Benjamin Orlando@hms.harvard.edu.

2

FREALIGN Using Samfrealign.py | Liao Lab

http://liao.hms.harvard.edu/node/30

From Micrographs to Particle Stack (Preprocessing). Particle Coordinate File formats. Defocus Determination Using Samdefocus.py. 2D classification and particle screening. FREALIGN Using Samfrealign.py. Preparation of Negative Stain Solution and EM Grids. SPIDER 3D Projection Matching Using Samrefine.py. Simple Steps for T12 Scope. FREALIGN Using Samfrealign.py. FREALIGN Using Samfrealign.py. Visit FREALIGN website: http:/ emlab.rose2.brandeis.edu/frealign. PLEASE CHECK GENERAL SETTINGS. Particle MRC imag...

3

SamViewer | Liao Lab

http://liao.hms.harvard.edu/samviewer

From Micrographs to Particle Stack (Preprocessing). Particle Coordinate File formats. Defocus Determination Using Samdefocus.py. 2D classification and particle screening. FREALIGN Using Samfrealign.py. Preparation of Negative Stain Solution and EM Grids. SPIDER 3D Projection Matching Using Samrefine.py. Simple Steps for T12 Scope. Is a 2D image display and analysis program. Specifically designed for single-particle EM. Is written in wxPython. Using Python Image Library(PIL) and Numpy. Works on both Linux.

4

Nanodisc Formation | Liao Lab

http://liao.hms.harvard.edu/node/34

From Micrographs to Particle Stack (Preprocessing). Particle Coordinate File formats. Defocus Determination Using Samdefocus.py. 2D classification and particle screening. FREALIGN Using Samfrealign.py. Preparation of Negative Stain Solution and EM Grids. SPIDER 3D Projection Matching Using Samrefine.py. Simple Steps for T12 Scope. More information about nanodiscs: http:/ sligarlab.life.uiuc.edu/nanodisc.html. Modified from a protocol originally developed by Saskia Neher, and provided by David Booth.).

5

Preparation of Negative Stain Solution and EM Grids | Liao Lab

http://liao.hms.harvard.edu/node/32

From Micrographs to Particle Stack (Preprocessing). Particle Coordinate File formats. Defocus Determination Using Samdefocus.py. 2D classification and particle screening. FREALIGN Using Samfrealign.py. Preparation of Negative Stain Solution and EM Grids. SPIDER 3D Projection Matching Using Samrefine.py. Simple Steps for T12 Scope. Preparation of Negative Stain Solution and EM Grids. Preparation of Negative Stain Solution and EM Grids. Prepare negative stain solution. And stir for another 5 min in the dark.

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Maofu Liao | Department of CELL BIOLOGY

http://cellbio.med.harvard.edu/people/faculty/liao

Skip to main content. Shao, Sichen (Susan). Van Vactor, David. How to Apply for an Internship. Cell Biology/BCMP Seminar Series. Cell Bio and Disease Seminar Series. Other Cell Bio Events. 2016 CDB Annual Spring Symposium. Past Cell Biology Symposia. 2016 Annual Cell Biology Retreat. Shao, Sichen (Susan). Van Vactor, David. Assistant Professor of Cell Biology. Department of Cell Biology. Boston, MA 02115. Http:/ liao.hms.harvard.edu/.

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Home | Liao Lab

From Micrographs to Particle Stack (Preprocessing). Particle Coordinate File formats. Defocus Determination Using Samdefocus.py. 2D classification and particle screening. FREALIGN Using Samfrealign.py. Preparation of Negative Stain Solution and EM Grids. SPIDER 3D Projection Matching Using Samrefine.py. Simple Steps for T12 Scope. We use high-resolution cryo-electron microscopy (cryo-EM) to study the structure and function of membrane proteins and DNA-protein machines. We moved to SGM-509!

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