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Biolab

Friday, July 28, 2006. Effect of single mismatches at 3' end of primers on polymerase chain reaction. Http:/ www.squ.edu.om/mj/Archive/Jan2000/simsek/index.html. We have studied here the priming efficiency of a 20 nucleotide long primer set which provided three different single mismatches (G/T, G/A and G/G) with the template DNA used. The G residue was located in the template (β-globin gene) while T, A or G were at the 3 end of the primers. Mismatched primer (figure 2, lane 4) contradict some of the publ...

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Biolab | mcb103.blogspot.com Reviews

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Friday, July 28, 2006. Effect of single mismatches at 3' end of primers on polymerase chain reaction. Http:/ www.squ.edu.om/mj/Archive/Jan2000/simsek/index.html. We have studied here the priming efficiency of a 20 nucleotide long primer set which provided three different single mismatches (G/T, G/A and G/G) with the template DNA used. The G residue was located in the template (β-globin gene) while T, A or G were at the 3 end of the primers. Mismatched primer (figure 2, lane 4) contradict some of the publ...

<META> KEYWORDS

1 biolab

2 abstract

3 discussion

4 or t/g

5 uote a a

6 错配使产量下降至1/20， a g

7 和 c c

8 引物a 模板g与引物g 模板a错配对pcr影响是等同的

9 0 comments

10 pcr引物设计原则zz

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biolab,abstract,discussion,or t/g,uote a a,错配使产量下降至1/20， a g,和 c c,引物a 模板g与引物g 模板a错配对pcr影响是等同的,0 comments,pcr引物设计原则zz,因此，引物的优劣直接关系到pcr的特异性与成功与否,要设计引物首先要找到dna序列的保守区,同时应预测将要扩增的片段单链是否形成二级结构,如这个区域单链能形成二级结构，就要避开它,如这一段不能形成二级结构，那就可以在这一区域设计引物,现在可以在这一保守区域里设计一对引物

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Biolab | mcb103.blogspot.com Reviews

https://mcb103.blogspot.com

Friday, July 28, 2006. Effect of single mismatches at 3' end of primers on polymerase chain reaction. Http:/ www.squ.edu.om/mj/Archive/Jan2000/simsek/index.html. We have studied here the priming efficiency of a 20 nucleotide long primer set which provided three different single mismatches (G/T, G/A and G/G) with the template DNA used. The G residue was located in the template (β-globin gene) while T, A or G were at the 3 end of the primers. Mismatched primer (figure 2, lane 4) contradict some of the publ...

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mcb103.blogspot.com

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Biolab: Stratagene Quikchange Primer Tm Calculator

http://mcb103.blogspot.com/2006/07/stratagene-quikchange-primer-tm.html

Friday, July 28, 2006. Stratagene Quikchange Primer Tm Calculator. Http:/ www.stratagene.com/QPCR/tmCalc.aspx. Posted by anoia at 3:40 AM. Los Angeles, CA, United States. View my complete profile. PCR引物设计原则zz.

2

Biolab: Effect of single mismatches at 3'–end of primers on polymerase chain reaction

http://mcb103.blogspot.com/2006/07/effect-of-single-mismatches-at-3end-of.html

Friday, July 28, 2006. Effect of single mismatches at 3' end of primers on polymerase chain reaction. Http:/ www.squ.edu.om/mj/Archive/Jan2000/simsek/index.html. We have studied here the priming efficiency of a 20 nucleotide long primer set which provided three different single mismatches (G/T, G/A and G/G) with the template DNA used. The G residue was located in the template (β-globin gene) while T, A or G were at the 3 end of the primers. Mismatched primer (figure 2, lane 4) contradict some of the publ...

3

Biolab: PCR引物设计原则zz

http://mcb103.blogspot.com/2006/07/pcrzz.html

Friday, July 28, 2006. 实验表明，待扩区域自由能 G 小于58.6lkJ/mol时，扩增往往不能成功。引物的有效长度 Ln=2 G C A T ，Ln值不能大于38，因为 38时，最适延伸温度会超过Taq DNA聚合酶的最适温度 74 ),不能保证产物的特异性。若按公式Tm=4 G C 2 A T 估计引物的Tm值，则有效引物的Tm为55 80 ，其Tm值最好接近72 以使复性条件最佳。 3 端也不能有形成任何二级结构可能，除在特殊的PCR AS-PCR 反应中，引物3 端不能发生错配。在标准PCR反应体系中，用2U Taq DNA聚合酶和800μmol/L dNTP 四种dNTP各200μmol/L 以质粒 103拷贝为模板，按95 ，25s 55 ，25s 72 ，1min的循环参数扩增HIV-1 gag基因区的条件下，引物3 端错配对扩增产物的影响是有一定规律的。 A A错配使产量下降至1/20，A G和C C错七下降至1/100。 Posted by anoia at 3:36 AM. Keep working. thnx!

4

Biolab: July 2006

http://mcb103.blogspot.com/2006_07_01_archive.html

Friday, July 28, 2006. Effect of single mismatches at 3' end of primers on polymerase chain reaction. Http:/ www.squ.edu.om/mj/Archive/Jan2000/simsek/index.html. We have studied here the priming efficiency of a 20 nucleotide long primer set which provided three different single mismatches (G/T, G/A and G/G) with the template DNA used. The G residue was located in the template (β-globin gene) while T, A or G were at the 3 end of the primers. Mismatched primer (figure 2, lane 4) contradict some of the publ...

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Biolab

Friday, July 28, 2006. Effect of single mismatches at 3' end of primers on polymerase chain reaction. Http:/ www.squ.edu.om/mj/Archive/Jan2000/simsek/index.html. We have studied here the priming efficiency of a 20 nucleotide long primer set which provided three different single mismatches (G/T, G/A and G/G) with the template DNA used. The G residue was located in the template (β-globin gene) while T, A or G were at the 3 end of the primers. Mismatched primer (figure 2, lane 4) contradict some of the publ...

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