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Our laboratory uses the Drosophila ovary as a model for studying the fundamental properties of epithelial stem cells, their associated niche, and the connection between epithelial stem cells and cancer. We are interested in questions such as:. How is stem cell fate maintained within a dynamic epithelial tissue? How do epithelial stem cells differ from other cells in the tissue? What is the nature of the epithelial stem cell niche? How is a healthy population of stem cells maintained throughout adult life?

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Our laboratory uses the Drosophila ovary as a model for studying the fundamental properties of epithelial stem cells, their associated niche, and the connection between epithelial stem cells and cancer. We are interested in questions such as:. How is stem cell fate maintained within a dynamic epithelial tissue? How do epithelial stem cells differ from other cells in the tissue? What is the nature of the epithelial stem cell niche? How is a healthy population of stem cells maintained throughout adult life?

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1 research interests

2 research

3 lab members

4 publications

5 microscope reservations

6 protocols

7 positions avaliable

8 fly stocks database

9 dept of anatomy

10 dept of ob/gyn rs

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research interests,research,lab members,publications,microscope reservations,protocols,positions avaliable,fly stocks database,dept of anatomy,dept of ob/gyn rs,bms graduate program,tetrad graduate program,dscb graduate program,lab news,read it here

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Home | nystullab.ucsf.edu Reviews

https://nystullab.ucsf.edu

Our laboratory uses the Drosophila ovary as a model for studying the fundamental properties of epithelial stem cells, their associated niche, and the connection between epithelial stem cells and cancer. We are interested in questions such as:. How is stem cell fate maintained within a dynamic epithelial tissue? How do epithelial stem cells differ from other cells in the tissue? What is the nature of the epithelial stem cell niche? How is a healthy population of stem cells maintained throughout adult life?

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nystullab.ucsf.edu

1

Protocols

http://nystullab.ucsf.edu/index.php/protocols

Retrospective genotyping (by PCR, lethality, or infertility). Stem cell replacement assay. Inverse PCR (Protocol from GDP). Standard ovary dissections and immunofluorescence. Optimizing fixation for a new antibody. PERK staining in the germarium. DNA extraction for single fly PCR. Bradford assay for protein quantitation. Last modified: 17 November 2016.

2

Positions Avaliable

http://nystullab.ucsf.edu/index.php/positions-avaliable

There are no positions available at this time, but please check back for updates in the fall. Applications for post-doctoral fellowship positions will be considered on a case-by-case basis. Please send your CV, a summary of your research accomplishments and interests, and the date you expect to become available to Todd Nystul (todd.nystul [at] ucsf.edu). Last modified: 17 November 2016.

3

Publications

http://nystullab.ucsf.edu/index.php/publications

Phosphorylated Groucho delays differentiation in the follicle stem cell lineage by providing a molecular memory of EGFR signaling in the niche. Johnston MJ, Cohen S, Paroush Z, Nystul TG. Increased intracellular pH is necessary for adult epithelial and embryonic stem cell differentiation. Ulmschneider B, Grillo-Hill BK,Benitez M,Azimova DR, Barber DL, Nystul TG. Journal of Cell Biology. 2016 Nov, 215 (3) 345-355. Vlachos S, Jangam S, Conder R, Chou M, Nystul T, Harden N. 2015 Jan 1;142(1):82-91. Drosophi...

4

Fly Stocks Database

http://nystullab.ucsf.edu/index.php/fly-stocks-database

To access the Fly Stocks Database, click here. To upload your stocks as a batch:. Download this zip file: DatabaseCSVs. Open the upload stocks.csv file in Excel and add your stock data. Perform a Find/Replace to remove all single apostrophes (') from your data. Fill in any empty fields (e.g. with the word none). Note, you can do this using the autofill function in Excel by typing the word none in the first row and then dragging the lower right corner of the cell down to the last row in your table. Log in...

5

Microscope Reservations

http://nystullab.ucsf.edu/index.php/microscope-reservations

Please use the Biological Imaging Development Center Scheduler. To reserve time on the CRS Apotome. To create a reservation, you will need a personal account in the BIDC system that has been granted access to the calendar for this microscope. Information about the filter sets available for the CRS Apotome can be found here. To create an account:. Sign up online by clicking on "New User Sign Up" on the Scheduler Homepage. For the current version. Last modified: 17 November 2016.

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anatomy.ucsf.edu

Faculty

http://anatomy.ucsf.edu/faculty

University of California San Francisco. PhD Assistant Professor lab website. PhD Professor and Chair lab website. Nripendra Dhillon, MBBS, MS Lecturer. Annemarie Donjacour, PhD Assistant Adjunct Professor. Amber Fitzsimmons, PhD Assistant Adjunct Professor. Derek Harmon, PhD Assistant Adjunct Professor. PhD Professor and Vice Chair lab website. PhD Adjunct Professor lab website. MD, PhD Professor lab website. MD, PhD Professor lab website. PhD Assistant Professor lab website. MD, PhD Professor lab website.

klein.hms.harvard.edu

Resources | Klein Lab

http://klein.hms.harvard.edu/resources

Skip to main content. Department of Systems Biology. 200 Longwood Avenue - Alpert 536. Boston, MA 02115. This page includes links to downloads, such as data and software that we’ve developed or use regularly in the lab. It also includes links to our collaborators, who make our interdisciplinary projects possible, and to some web resources we have found useful. Useful Databases and Bioinformatic Tools:. Cell-cycle dependent mRNA transcripts. BioMart gene symbol converter. Random DNA Sequence generator.

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nystullab.ucsf.edu

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Our laboratory uses the Drosophila ovary as a model for studying the fundamental properties of epithelial stem cells, their associated niche, and the connection between epithelial stem cells and cancer. We are interested in questions such as:. How is stem cell fate maintained within a dynamic epithelial tissue? How do epithelial stem cells differ from other cells in the tissue? What is the nature of the epithelial stem cell niche? How is a healthy population of stem cells maintained throughout adult life?

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