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Annealing Buffer 2X, 1 mL pH 8.3. Denhardt’s solution 100X, 50ml. DNA labeling buffer 10X, 1mL. EDTA 0.5M, 1 L pH 8.0. HEPES buffer, 1L pH 7.4. Laemmli buffer 6X , 10ml. LB Agar (for plates), 1L pH 7.5. LB Broth, 1L pH 7.5. Maleic Acid Buffer (MAB) 5X, 1L pH 7.5. Orange G Agarose gel loading buffer 6X, 10 ml. Paraformaldehyde 4%, 100ml pH 7.4. PBS 10X, 1L pH 7.4. TBE 5X, 2L. Tris 1M, 1L.

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Annealing Buffer 2X, 1 mL pH 8.3. Denhardt’s solution 100X, 50ml. DNA labeling buffer 10X, 1mL. EDTA 0.5M, 1 L pH 8.0. HEPES buffer, 1L pH 7.4. Laemmli buffer 6X , 10ml. LB Agar (for plates), 1L pH 7.5. LB Broth, 1L pH 7.5. Maleic Acid Buffer (MAB) 5X, 1L pH 7.5. Orange G Agarose gel loading buffer 6X, 10 ml. Paraformaldehyde 4%, 100ml pH 7.4. PBS 10X, 1L pH 7.4. TBE 5X, 2L. Tris 1M, 1L.
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https://recipes.labguru.com

Annealing Buffer 2X, 1 mL pH 8.3. Denhardt’s solution 100X, 50ml. DNA labeling buffer 10X, 1mL. EDTA 0.5M, 1 L pH 8.0. HEPES buffer, 1L pH 7.4. Laemmli buffer 6X , 10ml. LB Agar (for plates), 1L pH 7.5. LB Broth, 1L pH 7.5. Maleic Acid Buffer (MAB) 5X, 1L pH 7.5. Orange G Agarose gel loading buffer 6X, 10 ml. Paraformaldehyde 4%, 100ml pH 7.4. PBS 10X, 1L pH 7.4. TBE 5X, 2L. Tris 1M, 1L.

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RecipeDB

http://recipes.labguru.com/recipes/orange-g-agarose-gel-loading-buffer-6x-10-ml

Orange G Agarose gel loading buffer 6X, 10 ml. Http:/ openwetware.org/wiki/Agarose gel loading buffer. Store in small aliquots at 4 C or rt. Stock MW or Conc. Add very small amounts of Orange G dye gradually until the loading dye is dark orange. To use, add and mix loading dye and DNA solutions in a 1:5 ratio prior to loading into the wells of gels. Copy to my Labguru.

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RecipeDB

http://recipes.labguru.com/recipes/tbe-5x-2l

TBE 5X, 2L. The Weatherbee Lab, Yale University. Http:/ www.yale.edu/weatherbeelab/Weatherbee Lab/5X TBE.html. Stock MW or Conc. Add the above ingredients to 1800ml ddH2O. Adjust volume to 2L with additional ddH2O. Copy to my Labguru.

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http://recipes.labguru.com/recipes/lb-broth-1l-ph-7-5

LB Broth, 1L pH 7.5. Http:/ www.yale.edu/weatherbeelab/Weatherbee Lab/Luria Broth.html. Stock MW or Conc. Combine the ingredients above. Adjust pH to 7.5 with NaOH. Adjust volume to 1L with ddH2O. Copy to my Labguru.

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RecipeDB

http://recipes.labguru.com/recipes/laemmli-buffer-6x-10ml

Laemmli buffer 6X , 10ml. Easy Protocols: laboratory protocols and resources for your daily research. Http:/ www.easyprotocols.com/6x-laemmli-buffer-sample-buffer-for-western-blot/. Stock MW or Conc. Laemmli is a sample buffer to use in western blot. 5µl of protein sample 1µl of 6X Laemmli buffer is boiled or heated for 5-10 minutes. Warm all the ingredients except the DTT and shake it till everything is dissolved. (Takes time). Make sure everything is dissolved; then aliquot and freeze.

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http://recipes.labguru.com/recipes/lb-agar-for-plates-1l-ph-7-5

LB Agar (for plates), 1L pH 7.5. Http:/ www.yale.edu/weatherbeelab/Weatherbee Lab/Luria Agar.html. Stock MW or Conc. Add the Bacto-tryptone, NaCl to the ddH2O. Adjust pH to 7.5 with NaOH. Add the 15g of agar. Melt agar into solution in the microwave. Adjust volume to 1L with ddH2O. Add antibiotic if required. Pour into sterile petri dishes (approx. 20 ml/plate). Copy to my Labguru.

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Recipes | Labguru

https://www.labguru.com/features/knowledgebase/recipes

Add or Import Recipes for your Lab's Solutions. Labguru automatically recalculates volumes and weights based on your input. Researchers can add their solution and materials' recipes to their Labguru account, including:. Each ingredient's concentration, volume, weight, and source/vendor. As with all entities in Labguru, each recipe can be linked to its related protocols, experiment, project, storage location, and more. Video: How to Create Your Own Recipe In Labguru. Go to recipes.labguru.com.

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Annealing Buffer 2X, 1 mL pH 8.3. Denhardt’s solution 100X, 50ml. DNA labeling buffer 10X, 1mL. EDTA 0.5M, 1 L pH 8.0. HEPES buffer, 1L pH 7.4. Laemmli buffer 6X , 10ml. LB Agar (for plates), 1L pH 7.5. LB Broth, 1L pH 7.5. Maleic Acid Buffer (MAB) 5X, 1L pH 7.5. Orange G Agarose gel loading buffer 6X, 10 ml. Paraformaldehyde 4%, 100ml pH 7.4. PBS 10X, 1L pH 7.4. TBE 5X, 2L. Tris 1M, 1L.

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