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RevGenUK - Reverse Genetics, TILLING, mutation discovery : revgenuk.jic.ac.uk

RevGenUK (Reverse Genetics) is a TILLING service offered by the John Innes Centre to the plant science research community.

http://revgenuk.jic.ac.uk/

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RevGenUK - Reverse Genetics, TILLING, mutation discovery : revgenuk.jic.ac.uk | revgenuk.jic.ac.uk Reviews
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RevGenUK (Reverse Genetics) is a TILLING service offered by the John Innes Centre to the plant science research community.
<META>
KEYWORDS
1 what we offer
2 search databases
3 our populations
4 tilling process
5 wwwjic ac uk
6 how it works
7 search our databases
8 brassica rapa
9 re sequenced lines
10 revgen
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what we offer,search databases,our populations,tilling process,wwwjic ac uk,how it works,search our databases,brassica rapa,re sequenced lines,revgen,b rapa,b napus,cabriolet,b oleracea,capsella rubella,lotus japonicus,gifu,l japonicus,medicago truncatula
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RevGenUK - Reverse Genetics, TILLING, mutation discovery : revgenuk.jic.ac.uk | revgenuk.jic.ac.uk Reviews

https://revgenuk.jic.ac.uk

RevGenUK (Reverse Genetics) is a TILLING service offered by the John Innes Centre to the plant science research community.

INTERNAL PAGES

revgenuk.jic.ac.uk revgenuk.jic.ac.uk
1

RevGenUK - What We Offer - Reverse Genetics, TILLING : revgenuk.jic.ac.uk

http://revgenuk.jic.ac.uk/about

We offer a bespoke TILLING service on our populations of mutagenized model and crop species. We also have populations of re-sequenced lines (with suffix RS) and for these all you have to do is search the database for mutations in your gene of interest. You can then request these and we will send you seeds of up to five lines for a small handling charge 250 (academic rate). For full details of costs, policies and terms and conditions see our ‘Service costs and documentation’ page.

2

RevGenUK - Our populations - Reverse Genetics, TILLING : revgenuk.jic.ac.uk

http://revgenuk.jic.ac.uk/species

We have two populations for. The first raised in 2001-3 of individual lines representing 4904 families of ecotype Gifu B-129 and the second, 3652 lines of Miyakojima MG-20 produced in 2009-12. Perry J, Welham T, Brachmann A, Binder A, Charpentier M, Groth M, Haage K, Markmann K, Wang Tl, Parniske M. TILLING in. Identified large allelic series for symbiosis genes and revealed a bias in functionally defective ethyl methanesulfonate alleles towards glycine replacements. Plant Physiology 151. Accession we us...

3

Service costs and documentation - revgenuk.jic.ac.uk : revgenuk.jic.ac.uk

http://revgenuk.jic.ac.uk/service-costs-and-documentation

Service costs and documentation. Service costs and documentation. We currently (2015) charge 3100 ( VAT if applicable) to academic organisations for a conventional TILL (up to 1.2kb of genomic sequence) including primer design, production, and testing,. Costs of delivery and certification of seeds (i.e. phytosanitary certificates if needed). For sending you seeds from our exome captured and re-sequenced lines, we charge a 250 handling fee for up to five lines. Research only Materials Transfer Agreement.

4

RevGenUK - TILLING Process Explained - Reverse Genetics : revgenuk.jic.ac.uk

http://revgenuk.jic.ac.uk/process-explained

The technology, known as TILLING (Targeting Induced Local Lesions IN Genomes) is a ‘reverse genetics’ process as developed by Colbert et. al. 2001. The TILLING process explained…. 2: Growing the TILLING population. The M1 plants are self-fertilized and the M2 seed harvested and sown. The M2 germplasm will allow recessive and lethal alleles to be recovered as heterozygotes. Each M2 plant is given a unique identifier. 5: Pooling the DNA and amplification of Your Favourite Gene. To increase throughput the M...

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LINKS TO THIS WEBSITE

ukbrc.org.uk ukbrc.org.uk

Useful Resources | UK-BRC

http://ukbrc.org.uk/links-and-resources/other-useful-links

May 2015 Annual meeting – York. May 2014 Annual Meeting – Warwick, Wellesbourne. 2013 Annual Meeting – Rothamsted Research. April 2012 Annual Meeting – John Innes Cente. May 2011 Annual Meeting. May 2010 Annual Meeting. May 2009 Annual Meeting. May 2008 Annual Meeting. May 2007 Annual Meeting. Is a technology platform for reverse genetics in plants and is based at the John Innes Centre. They currently offer TILLING in Brassica rapa. R-o-18), Brassica napus. Cabriolet) and Brassica oleracea. The Arabidops...

brassica.info brassica.info

brassica.info | SITE MAP

http://www.brassica.info/sitemap.php

Fodder brassica - USA. Carleton College online lab. Brassica.info Bulletin (old). Guide to Wild Germplasm. UK plant science federation. Ensembl with Brassica tracks. Brassica crop and end-uses. Fodder brassica - USA. Guide to Wild Germplasm. Southern Cross Plant Science. Indicates external links (. We can not be held responsible for the content of external sites. ABN : 41 995 651 524 CRICOS Provider: 01241G.

brassica.info brassica.info

brassica.info - NUTRITIONAL BENEFITS

http://www.brassica.info/info/crop-enduses/nutritional-benefits.php

Fodder brassica - USA. Carleton College online lab. Brassica.info Bulletin (old). Guide to Wild Germplasm. UK plant science federation. Brassica Crop and end uses. Brassica crop and end-uses. Fodder brassica - USA. Guide to Wild Germplasm. Health, Medical and Clinical benefits of Brassica. Prepared by Graham King, Guy Barker HRI-Wellesbourne. Nov 2003 (re formatted Jan 2008). Are ranked as the leading causes of death in most industrialised countries. Vegetables appear to be especially protective. Plants ...

brassica.info brassica.info

brassica.info | BMAP

http://www.brassica.info/resource/sequencing/bmap.php

Fodder brassica - USA. Carleton College online lab. Brassica.info Bulletin (old). Guide to Wild Germplasm. UK plant science federation. Ensembl with Brassica tracks. Brassicales Map Alignment Project (BMAP). These pages have been set up to hold information (including notes from meetings, a list of target species etc.) for the Brassicales Map Alignment Project. BMAP) BMAP is analagous to a similar initiative that has been established for. Jan 2010 ( Minutes of meeting. Wuhan, June 2010 ( Minutes of meeting.

brassica.info brassica.info

brassica.info

http://www.brassica.info/resource/karyotypes.php

Fodder brassica - USA. Carleton College online lab. Brassica.info Bulletin (old). Guide to Wild Germplasm. UK plant science federation. Karyotyping of chromosomes within the genus Brassica. Has driven key stages in the development of our knowlegde of the evolution, radiation and domestication of this important taxa. Key advances have been made by the following:. U /Nagaharu (Woo Hang-choon). And peculiar mode of fertilization". Japan. J. Bot 7: 389 452. Southern Cross Plant Science.

brassica.info brassica.info

brassica.info

http://www.brassica.info/phenome.php

Fodder brassica - USA. Carleton College online lab. Brassica.info Bulletin (old). Guide to Wild Germplasm. UK plant science federation. Characterisation of all aspects of Brassica. Different levels and scale of data. Morphoplogical, phenological and associated agronomic traits. Ionomics - mineral variation. Southern Cross Plant Science. Indicates external links (. We can not be held responsible for the content of external sites. ABN : 41 995 651 524 CRICOS Provider: 01241G.

brassica.info brassica.info

brassica.info | ionomics

http://www.brassica.info/resource/ionomics.php

Fodder brassica - USA. Carleton College online lab. Brassica.info Bulletin (old). Guide to Wild Germplasm. UK plant science federation. Ensembl with Brassica tracks. Variation in plant element composition. The ionome is defined as the elemental composition of an organism. Purdue Ionomics Information Management System. Paper in Plant Physiology. Paper describing variation of Ca and Mg in. Broadley et al., 2008). This Page Was Last Edited:. Southern Cross Plant Science. Indicates external links (.

brassica.info brassica.info

brassica.info | Brassica genome sequencing

http://www.brassica.info/resource/sequencing.php

Fodder brassica - USA. Carleton College online lab. Brassica.info Bulletin (old). Guide to Wild Germplasm. UK plant science federation. DNA sequences and genome sequencing. 2014 saw the culmination of a major effort worldwide to generate 'reference' Brassica. Genome sequences, and these are now available online. The current focus is on a range of 're-sequencing' efforts. An early summary is presented below, but as of 2015 requires update. Please let send any relevant information to Graham King. Universit...

brassica.info brassica.info

brassica.info | proteomics

http://www.brassica.info/resource/proteomics.php

Fodder brassica - USA. Carleton College online lab. Brassica.info Bulletin (old). Guide to Wild Germplasm. UK plant science federation. Ensembl with Brassica tracks. Determining the protein composition of brassicas. At present, there are few proteomic datasets described or available for. Species. An initial study was carried out in the lab of Kathryn Lilley at University of Cambridge. Seed filling proteomic analysis. Interactome for B. rapa. This Page Was Last Edited:. Southern Cross Plant Science.

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RevGenUK - Reverse Genetics, TILLING, mutation discovery : revgenuk.jic.ac.uk

What information we need from you. Service costs and documentation. BLAST our database of TILLed genes. Search by gene name, ID, population or list all. To order a TILL. To request more information. Reverse Genetics – TILLING. UK is a TILLING service offered by the John Innes Centre to the plant science research community. We find mutations in your favourite gene. We currently offer TILLING in our Brassicaceae populations (. And in the model legumes. The John Innes Centre. In 2015, the platform was integ...

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