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Шлимовичи всех стран соединяйтесь! Земля, ты слышишь этот зуд? По тебе Шлимовичи ползут! 2013 Vladimir Shlimovich Для писем.

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Tuesday, May 22, 2007. Primary Culture of Mouse Dorsal root ganglian. Isolate mouse DRG and place into 15 ml DMEM (serum-free) solution. Centrifuge using 800rpm, at room temperature, for 2 minutes and then remove supernatant. Add 1 ml DMEM (serum-free) containing 0.125% Type Ia Collagenase (Stock=2.5%, take 50 ul into 950 ul DMEM), and incubate in 37 0C for 1.5 hours. Add 5 ml DMEM, and then gentled mix for a while. Centrifuge at 800rpm, RT for 2 minutes and then remove supernatant. Sunday, April 29, 2007.

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