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Simpson Lab Blog · SimpsonLab blog

Supporting R9 data in nanopolish. Two weeks ago I mentioned on twitter that I pushed a large series of changes to support R9 Oxford Nanopore data in nanopolish. I promised a blog post describing the changes then went off on my summer vacation - here it is, a little late. First, some background about R9 data. Back in March Clive Brown held a google hangout. On github contains instructions for using the new consensus workflow. ONT provided my lab with a batch of the latest flowcells. So far we have mostly ...

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Simpson Lab Blog · SimpsonLab blog | simpsonlab.github.io Reviews
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Supporting R9 data in nanopolish. Two weeks ago I mentioned on twitter that I pushed a large series of changes to support R9 Oxford Nanopore data in nanopolish. I promised a blog post describing the changes then went off on my summer vacation - here it is, a little late. First, some background about R9 data. Back in March Clive Brown held a google hangout. On github contains instructions for using the new consensus workflow. ONT provided my lab with a batch of the latest flowcells. So far we have mostly ...
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1 simpson lab blog
2 archive
3 the readme
4 r9 polishing results
5 homopolymers
6 deprecating nanocorrect
7 which used daligner
8 and poa
9 and calling snps
10 and miniasm
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simpson lab blog,archive,the readme,r9 polishing results,homopolymers,deprecating nanocorrect,which used daligner,and poa,and calling snps,and miniasm,finding ebola snps,background,events,and here,the first algorithm,reference,snp haplotype,nanopore data
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Simpson Lab Blog · SimpsonLab blog | simpsonlab.github.io Reviews

https://simpsonlab.github.io

Supporting R9 data in nanopolish. Two weeks ago I mentioned on twitter that I pushed a large series of changes to support R9 Oxford Nanopore data in nanopolish. I promised a blog post describing the changes then went off on my summer vacation - here it is, a little late. First, some background about R9 data. Back in March Clive Brown held a google hangout. On github contains instructions for using the new consensus workflow. ONT provided my lab with a batch of the latest flowcells. So far we have mostly ...

INTERNAL PAGES

simpsonlab.github.io simpsonlab.github.io
1

Deprecating Nanocorrect

http://simpsonlab.github.io/2016/02/25/deprecating-nanocorrect

Feb 25, 2016. When Nick, Josh and I got together at the Newton Institute hackathon. In 2014 we wanted to see if assembling nanopore data was possible. Over the week we put together a pipeline inspired by pbdagcon. To correct sequencing errors in nanopore reads. This software, which we called nanocorrect. Improved the accuracy of our nanopore reads to around 97% after two rounds of correction. The corrected reads worked well in the Celera Assembler and we had very long contigs soon after the hackathon.

2

Finding Ebola SNPs

http://simpsonlab.github.io/2016/02/03/ebola-snps

Feb 3, 2016. Today Nature published our paper. Describing the use of the Oxford Nanopore MinION to track the Ebola outbreak in West Africa. Over on Nick Loman’s blog. You can read an excellent account of how this project started and progressed. For my part, I worked on the method that we used to call SNPs from the sequenced Ebola samples, which I’ll describe in this post. Nanopore sequencing measures the changes in electric current (termed. And in previous blog posts here. Last summer Nick and Matt Loose...

3

Nanopolish v0.4.0

http://simpsonlab.github.io/2015/10/07/nanopolish-v0.4.0

Nanopolish v0.4.0. Oct 7, 2015. This is a long overdue post describing recent changes to nanopolish. Last month Oxford Nanopore released a new sequencing kit, SQK006. As Nick notes. SQK006 also brings an increase in speed - from 30bp/s (SQK005) to 70bp/s (SQK006). With the sampling rate fixed to 3kHz we naturally expect more events to be missed by the event detector. We observed this in our analysis of SQK006 data and updated the initial transition probabilities in our HMM accordingly. Field has been rem...

4

Nanopolish v0.2.0

http://simpsonlab.github.io/2015/03/30/optimizing-hmm

Nanopolish v0.2.0. Mar 30, 2015. This post describes changes I have made to nanopolish. Our HMM-based consensus caller for Oxford Nanopore data. This post can be thought of as a long changelog with background and rationale. Josh Quick and I started working on nanpore assembly at a hackathon at the Newton Institute in Cambridge. Our initial goal was pretty simple; we wanted to see if we could run DALIGNER. I asked on twitter the best way to call out to a C library from Python and received many helpful rep...

5

Supporting R9 data in nanopolish

http://simpsonlab.github.io/2016/08/23/R9

Supporting R9 data in nanopolish. Aug 23, 2016. Two weeks ago I mentioned on twitter that I pushed a large series of changes to support R9 Oxford Nanopore data in nanopolish. I promised a blog post describing the changes then went off on my summer vacation - here it is, a little late. First, some background about R9 data. Back in March Clive Brown held a google hangout. On github contains instructions for using the new consensus workflow. ONT provided my lab with a batch of the latest flowcells. So far w...

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Supporting R9 data in nanopolish. Two weeks ago I mentioned on twitter that I pushed a large series of changes to support R9 Oxford Nanopore data in nanopolish. I promised a blog post describing the changes then went off on my summer vacation - here it is, a little late. First, some background about R9 data. Back in March Clive Brown held a google hangout. On github contains instructions for using the new consensus workflow. ONT provided my lab with a batch of the latest flowcells. So far we have mostly ...

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