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UT Knoxville | Department of Biochemistry & Cellular & Molecular Biology - Dr. Albrecht von Arnim's Lab Page

The University of Tennessee, Knoxville was founded in 1794 and was designated the state land-grant institution in 1879. The University now has nearly 26,000 students and 400 academic programs.

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UT Knoxville | Department of Biochemistry & Cellular & Molecular Biology - Dr. Albrecht von Arnim's Lab Page | vonarnimlab.utk.edu Reviews
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UT Knoxville | Department of Biochemistry & Cellular & Molecular Biology - Dr. Albrecht von Arnim's Lab Page | vonarnimlab.utk.edu Reviews

https://vonarnimlab.utk.edu

The University of Tennessee, Knoxville was founded in 1794 and was designated the state land-grant institution in 1879. The University now has nearly 26,000 students and 400 academic programs.

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vonarnimlab.utk.edu vonarnimlab.utk.edu
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UT Knoxville | Department of Biochemistry & Cellular & Molecular Biology - Dr. Albrecht von Arnim's Lab Page

http://vonarnimlab.utk.edu/vectors.shtml

Skip to Main Content. The University of Tennessee. Search The University of Tennessee:. Von Arnim Lab ». Raquo; BRET Vectors. Three sets of BRET vectors have been made (see Table. PBS-35S-RLUC and pBS-35S-YFP allow you to make plain fusions to either the N-terminus or C-terminus of RLUC (Renilla luciferase) or YFP (yellow fluorescent protein). Cite Subramanian et al., 2004. PNAS 101:6798-6802. Gateway vectors with and without T-DNA borders. Cite Subramanian et al., 2006. Plant Journal 48:138-152. Vectors...

2

UT Knoxville | Department of Biochemistry & Cellular & Molecular Biology - Dr. Albrecht von Arnim's Lab Page

http://vonarnimlab.utk.edu/default.html

Skip to Main Content. The University of Tennessee. Search The University of Tennessee:. Von Arnim Lab ». Albrecht von Arnim, Ph.D. Lab Page. The past ten years have yielded surprising insights into the role of RNA in gene regulation. Protein synthesis (translation) remains poorly understood, although translational control affords a rapid means to amplify changes that occur at the level of transcription. We are using the model system. 1988 - MS, University of Freiburg, Germany. Knoxville, TN 37996. Middot...

3

UT Knoxville | Department of Biochemistry & Cellular & Molecular Biology - Dr. Albrecht von Arnim's Lab Page

http://vonarnimlab.utk.edu/advice.shtml

Skip to Main Content. The University of Tennessee. Search The University of Tennessee:. Von Arnim Lab ». Raquo; BRET Advice. Please contact Albrecht von Arnim with requests for vectors, procedures, or advice. Use procedures at your own risk! Your goal should be to achieve immediate juxtaposition of the RLUC and YFP tags ( 5nm distance). With small proteins ( 10kDa), it may not matter which way you fuse the BRET tags. With medium sized proteins (50kDa) it will matter. Cell types: We have expressed BRET ta...

4

UT Knoxville | Department of Biochemistry & Cellular & Molecular Biology - Dr. Albrecht von Arnim's Lab Page

http://vonarnimlab.utk.edu/staff.shtml

Skip to Main Content. The University of Tennessee. Search The University of Tennessee:. Von Arnim Lab ». Raquo; Lab Staff. Dr Minviluz 'Bing' G. Stacey. Dr Olga R. Kopp. The University of Tennessee. Middot; Knoxville, TN 37996 · (865) 974-1000 · Contact UT.

5

UT Knoxville | Department of Biochemistry & Cellular & Molecular Biology - Dr. Albrecht von Arnim's Lab Page

http://vonarnimlab.utk.edu/bret.shtml

Skip to Main Content. The University of Tennessee. Search The University of Tennessee:. Von Arnim Lab ». Raquo; What is BRET? We are using the original BRET system described by the Johnson lab. Xu et al., 1999; PNAS 96:151-156), in which the blue light emitting Renilla luciferase. RLUC) is paired with Yellow fluorescent protein (YFP). The University of Tennessee. Middot; Knoxville, TN 37996 · (865) 974-1000 · Contact UT.

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UT Knoxville | Department of Biochemistry & Cellular & Molecular Biology - Dr. Albrecht von Arnim's Lab Page

Skip to Main Content. The University of Tennessee. Search The University of Tennessee:. Von Arnim Lab ». Albrecht von Arnim, Ph.D. Lab Page. The past ten years have yielded surprising insights into the role of RNA in gene regulation. Protein synthesis (translation) remains poorly understood, although translational control affords a rapid means to amplify changes that occur at the level of transcription. We are using the model system. 1988 - MS, University of Freiburg, Germany. Knoxville, TN 37996. Middot...

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