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CRISPR RGEN Tools
http://www.rgenome.net/about
RGENs, Cas-OFFinder, Microhomology-Predictor, Cas-Designer, and Cas-Database. In January 2013, several groups ( Cho et al. Independently reported a new class of genome editing nucleases termed RNA-guided endonucleases (RGENs) herein to avoid confusion with the original type II clustered regularly interspaced short palindromic repeat (CRISPR) Cas (CRISPR-associated) adaptive immune system in bacteria the specificity of which is mostly determined by small guide RNAs rather than by DNA-binding proteins....
CRISPR RGEN Tools
http://www.rgenome.net/cas-offinder
A fast and versatile algorithm that searches for potential off-target sites of Cas9 RNA-guided endonucleases. Citation info: Bae S., Park J., and Kim J.-S. Cas-OFFinder: A fast and versatile algorithm that searches for potential off-target sites of Cas9 RNA-guided endonucleases. Bioinformatics. Submit a new searching job, or download an off-line version of Cas-OFFinder here. CRISPR/Cas-derived RNA-guided Endonucleases (RGENs). SpCas9 from Streptococcus pyogenes: 5-NGG-3. FnCpf1 from Francisella: 5-TTN-3.
CRISPR RGEN Tools
http://www.rgenome.net/mich-calculator
Microhomology-based choice of Cas9 nuclease target sites. When programmable nucleases (such as ZFNs, TALENs, or CRISPR) are treated, 1 3bp deletion or 1bp insertion would be induced via. Citation info: Bae S. et al. Microhomology-based choice of Cas9 nuclease target sites. Nature Methods. Score of each pattern according to the microhomology size and the deletion length. The sum of all the pattern scores. The ratio of out-of-frame pattern scores over the sum of all the pattern scores. Insert any sequence ...
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