At the Cytokine Receptor Lab, we combine basic research with technology development. Using growing insights in cytokine receptor signalling, we are developing the MAPPIT toolbox, a technology platform that allows studying molecular interactions in intact mammalian cells.
http://www.crl-mappit.be/
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Home | CRL: Cytokine Receptor Lab | crl-mappit.be Reviews
https://crl-mappit.be
At the Cytokine Receptor Lab, we combine basic research with technology development. Using growing insights in cytokine receptor signalling, we are developing the MAPPIT toolbox, a technology platform that allows studying molecular interactions in intact mammalian cells.
crl-mappit.be
Pathway analysis | CRL: Cytokine Receptor Lab
http://www.crl-mappit.be/mappit_toolbox/mappit_applications/pathway_analysis
MAPPIT protocols and vectors. Walking the TLR4 pathway using binary ForwardMAPPIT assays. Ulrichts and Tavernier. MAPPIT analysis of early Toll-like receptor signalling events. Immunol. Lett. (2008). MAPPIT protocols and vectors.
Mappit applications | CRL: Cytokine Receptor Lab
http://www.crl-mappit.be/mappit_toolbox/mappit_applications
MAPPIT protocols and vectors. The MAPPIT technology platform can be applied in various fields. Examples are listed where MAPPIT is used in pathway analysis. MAPPIT protocols and vectors.
ReverseMAPPIT | CRL: Cytokine Receptor Lab
http://www.crl-mappit.be/mappit_toolbox/mappit_modes/reversemappit
MAPPIT protocols and vectors. Based on either STAT3 dependent reporter induction or direct measurement of STAT3 activation. Reverse MAPPIT: screening for protein-protein interaction modifiers in mammalian cells. Nat. Methods (2005). Reverse MAPPIT detects disruptors of protein-protein interactions in human cells. Nat. Protoc. (2006). MAPPIT protocols and vectors.
HeteromericMAPPIT | CRL: Cytokine Receptor Lab
http://www.crl-mappit.be/mappit_toolbox/mappit_modes/heteromericmappit
MAPPIT protocols and vectors. Heteromeric MAPPIT: a novel strategy to study modification-dependent protein-protein interactions in mammalian cells. Nucleic Acids Res. (2003). MAPPIT protocols and vectors.
ForwardMAPPIT | CRL: Cytokine Receptor Lab
http://www.crl-mappit.be/mappit_toolbox/mappit_modes/forwardmappit
MAPPIT protocols and vectors. In the basic lay-out of ForwardMAPPIT, the bait is fused to a signalling-deficient receptor chimera and the prey is tethered to a receptor fragment containing functional STAT3 recruitment sites. Bait-prey interaction reconstitutes a functional receptor complex which, upon ligand binding, initiates the JAK-STAT signalling cascade. For analysis of the interaction between designated protein pairs, the ForwardMAPPIT mode can be combined with a binary assay. Analysis of leptin si...
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Links to laboratories | Switch Laboratory
http://www.switchlab.org/links/labs
Mapping aggregation-specific interactions in human disease. FoldX plugin for YASARA. Protein folding and aggregation labs. Northwestern University, IL, USA. Cambridge University, UK. Scripps Institute, CA, USA. UCLA, CA, USA. Stanford University, CA, USA. Whitehead Institute, MIT, MA, USA. Stanford University, CA, USA. FRS, Cambridge University, UK. Stockholm University, Sweden. Cambridge University, UK. Cambridge University, UK. University of Pensylvania, PA, USA. KU Leuven, Belgium. KU Leuven, Belgium.
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Home | CRL: Cytokine Receptor Lab
MAPPIT protocols and vectors. CRL was founded in 1996 at Ghent University. Belgium, as a research group within the VIB. Department of Medical Protein Research. Our team of 35 researchers combines extensive cell biology and molecular biology expertise and is housed in recently renovated laboratory facilities at the historic Rommelaere Institute. As a technology spin-off, we develop the MAPPIT and KISS two-hybrid traps. More recently, we are developing AcTakines. MAPPIT protocols and vectors.
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