flypush.imgen.bcm.tmc.edu

fly-facility.com

Fly Facility

http://www.fly-facility.com/protocols/index.php

Using our services you can rapidly generate both P-element and site-specific transgenic Drosophila lines carrying different reporter gene constructs. And test gene function by dsRNA microinjection. More than 1000 transgenic lines. Generated and more than 200 dsRNAs. Injected and analysed. Our device : Quality, Rapidity and Competitive pricing. P-element transgenic line production. Injections are performed within 2 to 7 days upon arrival of the samples. Survival larvae are picked up in 48 h after injection.

pacmanfly.org

P[acman] Resources - Reagents

http://www.pacmanfly.org/reagents.html

Available from the Drosophila. Available from other sources. Available from BACPAC Resources. Available from the BDSC: see stocks page. Please send comments or questions about the website to Karen Schulze.

unihuaii.com

PhiC31整合系统_联合华益

http://www.unihuaii.com/Recruit/fuwuyujishulei

Http:/ genepath.med.harvard.edu/WuLab/RMCE. Http:/ flypush.imgen.bcm.tmc.edu/lab/index.html. Http:/ bicoid.lbl.gov/cgi-bin/gbrowse/getbac/. Https:/ bacpac.chori.org/library.php? Http:/ www.frontiers-in-genetics.org/flyc31/. Http:/ flypush.imgen.bcm.tmc.edu/pscreen/technique.html. Groth A, Fish M, Nusse R, Calos MP (2004). By using the site-specific integrase. Szabad J, Bellen HJ, Venken KJT (2012). An Assay to Detect In Vivo Y Chromosome Loss in. Bateman JR, Lee AM, Wu C-t (2006). Proc Natl Acad Sci USA.

thebestgene.com

BestGene Inc. - PhiC31 Info

http://www.thebestgene.com/PhiC31InfoPage.do

PhiC31 integrase-mediated transgenesis systems. PhiC31 integrase-mediated transgenesis systems are based on the site-specific bacteriophage PhiC31 integrase which mediates sequence-directed, irreversible and highly efficient integration between a bacterial attachment site ( attB. And a phage attachment site ( attP. Injecting plasmid containing attB. Site and white marker into attP. Containing docking site strain(s) with PhiC31 activity makes the resultant stable w. We use M{vas-int.Dm}ZH-2A. In June, 200...

lianhehuayi.com

PhiC31整合系统_联合华益

http://www.lianhehuayi.com/Recruit/fuwuyujishulei

Http:/ genepath.med.harvard.edu/WuLab/RMCE. Http:/ flypush.imgen.bcm.tmc.edu/lab/index.html. Http:/ bicoid.lbl.gov/cgi-bin/gbrowse/getbac/. Https:/ bacpac.chori.org/library.php? Http:/ www.frontiers-in-genetics.org/flyc31/. Http:/ flypush.imgen.bcm.tmc.edu/pscreen/technique.html. Groth A, Fish M, Nusse R, Calos MP (2004). By using the site-specific integrase. Szabad J, Bellen HJ, Venken KJT (2012). An Assay to Detect In Vivo Y Chromosome Loss in. Bateman JR, Lee AM, Wu C-t (2006). Proc Natl Acad Sci USA.

pacmanfly.org

P[acman] Resources - Protocols

http://www.pacmanfly.org/protocols.html

PDF file - download. Venken et al. (2006) Science. Venken et al. (2009) Nature Methods. For more ΦC31 tools, visit FlyC31. Please send comments or questions about the website to Karen Schulze.

pacmanfly.org

P[acman] Resources - About P[acman]

http://www.pacmanfly.org/pacman.html

Element termini and eye color marker allow it to be transposed directly into flies using P. Transposase. However, attB-P[acman]. Contains an attB site that allows permanent integration at specific docking points (these are equipped with the complementary attP sequence) that have been created in the genome (see Docking Sites. Page) using ΦC31 transposase. Due to the size limitations posed by P. Transposase-mediated transformation, ΦC31-mediated transgenesis is preferred for large constructs.

pacmanfly.org

P[acman] Resources - Libraries

http://www.pacmanfly.org/libraries.html

Two genomic BAC libraries (83 kb and 21 kb average clone size) have been generated and the clones are engineered into the attB-P[acman]-Cm. Vector, a special adaptation of P[acman]. Click below to search for clones. Venken, KJT, Carlson JW, Schulze KL, Pan H, He Y, Spokony R, Wan KH, Koriabine M, de Jong PJ, White KP, Bellen HJ, Hoskins RA (2009) Versatile P[acman] BAC Libraries for transgenesis studies in Drosophila melanogaster. Nature Methods. 6:431-434. [ Abstract. Table S3 ( PDF. Table S4 ( PDF.

pharmacology.mc.duke.edu

Duke University School of Medicine - Department of Pharmacology and Cancer Biology

http://pharmacology.mc.duke.edu/seminar/index.html

Duke University School of Medicine. Office of Research Administration. Duke University Integrated Toxicology Program. Duke University Small Molecule Synthesis Facility. DEPARTMENT OF PHARMACOLOGY and CANCER BIOLOGY. The Pharmacology and Cancer Biology seminar series Regulatory Networks in Health and Disease is held on Wednesdays at 12:00 PM in 103 Bryan Research Building - unless otherwise indicated. Trever Bivona, MD, PhD. Molecular strategies for enabling the evolution of precision oncology. Deconstruc...