pacmanfly.org

P[acman] Resources

This website is the source for information and resources of tools for the Drosophila. Community using the P[acman]. Recombineering technique. What is P[acman]. Click About for more information. Duplications for the X. Recombineering ready, mapped genomic BAC clones (see Libraries. Fly stocks containing stably integrated docking sites (see Docking Sites. Cloning tools for recombineering (see Vectors/Reagents. As more tools are developed we will add them to this site.

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P[acman] Resources | pacmanfly.org Reviews

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This website is the source for information and resources of tools for the Drosophila. Community using the P[acman]. Recombineering technique. What is P[acman]. Click About for more information. Duplications for the X. Recombineering ready, mapped genomic BAC clones (see Libraries. Fly stocks containing stably integrated docking sites (see Docking Sites. Cloning tools for recombineering (see Vectors/Reagents. As more tools are developed we will add them to this site.

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P[acman] Resources | pacmanfly.org Reviews

https://pacmanfly.org

This website is the source for information and resources of tools for the Drosophila. Community using the P[acman]. Recombineering technique. What is P[acman]. Click About for more information. Duplications for the X. Recombineering ready, mapped genomic BAC clones (see Libraries. Fly stocks containing stably integrated docking sites (see Docking Sites. Cloning tools for recombineering (see Vectors/Reagents. As more tools are developed we will add them to this site.

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pacmanfly.org

1

P[acman] Resources - Docking Sites

http://www.pacmanfly.org/docking.php

The stocks containing the docking sites are available for order from the. The information below reflects Release 6.01. Venken KJT, He Y, Hoskins RA, Bellen HJ (2006) P[acman]: a BAC transgenic platform for targeted insertion of large DNA fragments in D. melanogaster. Science. Y[1] w[1118]; PBac{y -attP}VK00001. Y[1] w[1118]; PBac{y -attP}VK00002. Y[1] w[1118]; PBac{y -attP}VK00003a; PBac{y -attP}VK00003b. Y[1] w[1118]; PBac{y -attP}VK00005. Y[1] w[1118] PBac{y -attP}VK00006. Y[1] w[1118]; PBac{y -attP}VK...

2

P[acman] Resources - Duplications

http://www.pacmanfly.org/dups.php

The stocks containing the duplications are available for order from the Bloomington Drosophila. The information below has been updated to Release 6.01. Venken KJ, Popodi E, Holtzman SL, Schulze KL, Park S, Carlson JW, Hoskins RA, Bellen HJ, Kaufman TC (2010) A molecularly defined duplication set for the X chromosome of Drosophila melanogaster. Genetics. Please send comments or questions about the website to Karen Schulze.

3

P[acman] Resources - About P[acman]

http://www.pacmanfly.org/pacman.html

Element termini and eye color marker allow it to be transposed directly into flies using P. Transposase. However, attB-P[acman]. Contains an attB site that allows permanent integration at specific docking points (these are equipped with the complementary attP sequence) that have been created in the genome (see Docking Sites. Page) using ΦC31 transposase. Due to the size limitations posed by P. Transposase-mediated transformation, ΦC31-mediated transgenesis is preferred for large constructs.

4

P[acman] Resources - Protocols

http://www.pacmanfly.org/protocols.html

PDF file - download. Venken et al. (2006) Science. Venken et al. (2009) Nature Methods. For more ΦC31 tools, visit FlyC31. Please send comments or questions about the website to Karen Schulze.

5

P[acman] Resources - Credits

http://www.pacmanfly.org/credits.html

The P[acman] library project is a joint collaboration of the laboratories of Hugo Bellen at Baylor College of Medicine and Roger Hoskins at Lawrence Berkeley National Laboratory. It is supported by the Howard Hughes Medical Institute and the National Institutes of Health. The Duplication Consortium members include:. Indiana University: Ellen Popodi, Stacey Holtzman, Melissa Phelps, Robert Eisman, Dave Miller and Thom Kaufman. Baylor College of Medicine: Koen Venken, Karen Schulze and Hugo Bellen.

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flyfacility.wordpress.com

flyfacility | flyfacility

https://flyfacility.wordpress.com/author/flyfacility

The University of Manchester is host to a large and diverse. Research community, currently comprising 13 groups which work on a wide range of different biological questions and are embedded in a vibrant research environment (for details see Faculty research domains. Along with our state of the art fly facility this community makes Manchester a great environment for conducting. BMP signalling: development, stem cell maintenance and cancer. 8216;s Faculty profile. 8216; research profile. February 17, 2017.

flyfacility.ls.manchester.ac.uk

Fly links | Manchester Fly Facility

http://www.flyfacility.ls.manchester.ac.uk/flylinks

In June 2015 a fly resource list. Was set up on the FlyWiki which includes all the below links. We therefore no longer update this page and refer to that list instead. News, meeting, conferences. Genome and transcriptome expression databases. Proteome and protein expression databases. Plasmid depositories and gene libraries. Outreach and science communication. University of Manchester – Fly Facility – For the public. 8211; A comprehensive description of concepts and available resources for. 8211; The obj...

fly-jedi.org

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https://www.fly-jedi.org/resources

Add Job or Publication. The Return of the JEDI: the second annual meeting of the Junior European Drosophila Investigators. Developmental Studies Hybridoma Bank. Antibodies against Drosophila antigens (and not only). A cyberspace guide to Drosophila development and metazoan evolution. Supercharged distribution of ImageJ (Fiji Is Just ImageJ) with lots of plugins geared towards. Transgenesis and genomic libraries. Transgenesis ready fosmid libraries for. BAC transgenesis for your gene of choice. Why Drosop...

flyfacility.wordpress.com

Fly Links | flyfacility

https://flyfacility.wordpress.com/2014/11/07/fly-links

In June 2015 a fly resource list. Was set up on the FlyWiki which includes all the below links. We therefore no longer update this page and refer to that list instead. News, meeting, conferences. Genome and transcriptome expression databases. Proteome and protein expression databases. Plasmid depositories and gene libraries. Outreach and science communication. University of Manchester – Fly Facility – For the public. 8211; A comprehensive description of concepts and available resources for. 8211; The obj...

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P[acman] Resources

This website is the source for information and resources of tools for the Drosophila. Community using the P[acman]. Recombineering technique. What is P[acman]. Click About for more information. Duplications for the X. Recombineering ready, mapped genomic BAC clones (see Libraries. Fly stocks containing stably integrated docking sites (see Docking Sites. Cloning tools for recombineering (see Vectors/Reagents. As more tools are developed we will add them to this site.

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