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Gene-Quantification.info - The reference in qPCR - Academic & Industrial Information Platform

Academic & industrial information platform for qPCR - The page describes and summarises all technical aspects involved in quantitative gene expression analysis using real-time qPCR & qRT-PCR.

http://www.gene-quantification.eu/

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Gene-Quantification.info - The reference in qPCR - Academic & Industrial Information Platform | gene-quantification.eu Reviews
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Academic & industrial information platform for qPCR - The page describes and summarises all technical aspects involved in quantitative gene expression analysis using real-time qPCR & qRT-PCR.
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1 pcr
2 qpcr
3 workshop
4 application
5 rt-pcr
6 symposium
7 miqe guidelines
8 android
9 ios
10 html5
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gene quantification content,classical block rt pcr,and relative quantification,rest,editor@gene quantification info,and higher versions
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Gene-Quantification.info - The reference in qPCR - Academic & Industrial Information Platform | gene-quantification.eu Reviews

https://gene-quantification.eu

Academic & industrial information platform for qPCR - The page describes and summarises all technical aspects involved in quantitative gene expression analysis using real-time qPCR & qRT-PCR.

INTERNAL PAGES

gene-quantification.eu gene-quantification.eu
1

BioInformatics & relative quantification using real timePCR

http://www.gene-quantification.eu/relative.html

Relative Quantification or Comparative Quantification in qPCR. Calculating the relative expression ratio. Relative quantification models WITHOUT efficiency correction. Relative quantification models WITH efficiency correction - using averaged mean PCR efficiency. Relative quantification models WITH efficiency correction - using single-run PCR efficiency. Go to efficiency correction page. Chapter 3: Relative Quantification. By Michael W. Pfaffl. In: Real-time PCR. (Editor: T. Dorak), p 63-82. Chini V, Fok...

2

Gene Quantification & real time or kinetic PCR / RT-PCRinternational literature

http://www.gene-quantification.eu/literature.html

Real-time PCR & qPCR literature. Last update in 2008. By M Tevlik Dorak. Last update in 2008. RT-PCR and real-time RT-PCR (in general). LightCycler specific technical notes. Absolute quantification using kinetic PCR. Relative quantification using kinetic PCR. Classical block RT-PCR & competitive RT-PCR. Normalisation & housekeeping gene problems in real-time PCR. Verification of DNA array results. Quantification software: REST, REST-XL, Q-Gene, geNorm, RTPrimerDB. Kinetic RT-PCR poster board. Welcome to ...

3

Relative expression software tool (REST)

http://www.gene-quantification.eu/rest.html

Relative Expression Software Tool = REST. Today the " relative. Is increasingly used in gene expression studies, where the expression of a target gene is standardised. By a non-regulated reference-gene or by an index, containing more reference-genes. Several mathematical algorithm have been d. Eveloped to compute the expression ratio, based on real-time PCR efficiency and the. Crossing point (Ct or CP) deviation (= delta CP) of an unknown sample versus a control. But all published equations. Y Vainshtein...

4

Gene Quantification & block RT-PCR

http://www.gene-quantification.eu/block.html

Polymerase Chain Reaction (PCR). Reverse Transcription - Polymerase Chain Reaction (RT-PCR). The Nobel Prize in Chemistry 1993. Polymerase Chain Reaction -. The PCR method – a copying machine for DNA molecules. Http:/ nobelprize.org/nobel prizes/chemistry/laureates/1993/illpres/pcr.html. Roche Applied Science - PCR Application Manual 3rd Edition. PCR and RT-PCR are techniques so common in today's laboratories that it is easy to forget its. Any given cycle that falls within the reaction's exponential range.

5

Posterboard A on Gene-Quantification.info

http://www.gene-quantification.eu/poster-a.html

Poster board A: Array verification. Most posters are in Adobe Acrobat Format ( PDF ). Using real-time PCR for cDNA Array verification. Birgit Kindermann, Hannelore Daniel, Michael W. Pfaffl and Frank Döring (2002). Einsatz der cDNA-Array-Technologie zur Identifizierung Zink-sensitiver Gene in-vitro. Dechema Statusseminar „Chiptechnologie: Transkriptom – Proteom – Metabolom“, S.79. Birgit Kindermann, Hannelore Daniel, Michael W. Pfaffl and Frank Döring (2002). Array Meeting: COST B20: Mammary development,...

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Gene-Quantification.info - The reference in qPCR - Academic & Industrial Information Platform

BioScience Events - Dr. Michael W. Pfaffl editor@gene.quantification.info. Main focus of the GENE QUANTIFICATION web page. Is, to describe and summarize all technical aspects. Involved in quantitative gene expression analysis using real-time (kinetic) PCR & RT-PCR. Eg detection chemistry, assay specificity, sensitivity, reproducibility, irobustness,. Intra- and inter-assay variations, kinetic PCR efficiency calculation, quantification strategies,. Main focus of the PHYSIOLOGY web page. Description of qua...

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BioScience Events - Dr. Michael W. Pfaffl editor@gene.quantification.info. Main focus of the GENE QUANTIFICATION web page. Is, to describe and summarize all technical aspects. Involved in quantitative gene expression analysis using real-time (kinetic) PCR & RT-PCR. Eg detection chemistry, assay specificity, sensitivity, reproducibility, irobustness,. Intra- and inter-assay variations, kinetic PCR efficiency calculation, quantification strategies,. Main focus of the PHYSIOLOGY web page. Description of qua...

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Gene-Quantification.info - The reference in qPCR - Academic & Industrial Information Platform

BioScience Events - Dr. Michael W. Pfaffl editor@gene.quantification.info. Main focus of the GENE QUANTIFICATION web page. Is, to describe and summarize all technical aspects. Involved in quantitative gene expression analysis using real-time (kinetic) PCR & RT-PCR. Eg detection chemistry, assay specificity, sensitivity, reproducibility, irobustness,. Intra- and inter-assay variations, kinetic PCR efficiency calculation, quantification strategies,. Main focus of the PHYSIOLOGY web page. Description of qua...

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Gene-Quantification.info - The reference in qPCR - Academic & Industrial Information Platform

BioScience Events - Dr. Michael W. Pfaffl editor@gene.quantification.info. Main focus of the GENE QUANTIFICATION web page. Is, to describe and summarize all technical aspects. Involved in quantitative gene expression analysis using real-time (kinetic) PCR & RT-PCR. Eg detection chemistry, assay specificity, sensitivity, reproducibility, irobustness,. Intra- and inter-assay variations, kinetic PCR efficiency calculation, quantification strategies,. Main focus of the PHYSIOLOGY web page. Description of qua...

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Gene-Quantification.info - The reference in qPCR - Academic & Industrial Information Platform

Gene-Quantification.info - The reference in qPCR - Academic and Industrial Information Platform - The page describes and summarises all technical aspects involved in quantitative gene expression analysis using real-time qPCR and qRT-PCR. It presents a lot of cyclers, kits, dyes, chemistries, and services involved. Companies and institutions can present their qPCR technologies, knowledge and services right here. - gene-quantification.info.

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Gene-Quantification.info - The reference in qPCR - Academic & Industrial Information Platform

BioScience Events - Dr. Michael W. Pfaffl editor@gene.quantification.info. Main focus of the GENE QUANTIFICATION web page. Is, to describe and summarize all technical aspects. Involved in quantitative gene expression analysis using real-time (kinetic) PCR & RT-PCR. Eg detection chemistry, assay specificity, sensitivity, reproducibility, irobustness,. Intra- and inter-assay variations, kinetic PCR efficiency calculation, quantification strategies,. Main focus of the PHYSIOLOGY web page. Description of qua...

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Gene-Quantification.info - The reference in qPCR - Academic & Industrial Information Platform

BioScience Events - Dr. Michael W. Pfaffl editor@gene.quantification.info. Main focus of the GENE QUANTIFICATION web page. Is, to describe and summarize all technical aspects. Involved in quantitative gene expression analysis using real-time (kinetic) PCR & RT-PCR. Eg detection chemistry, assay specificity, sensitivity, reproducibility, irobustness,. Intra- and inter-assay variations, kinetic PCR efficiency calculation, quantification strategies,. Main focus of the PHYSIOLOGY web page. Description of qua...

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