Academic & industrial information platform for qPCR - The page describes and summarises all technical aspects involved in quantitative gene expression analysis using real-time qPCR & qRT-PCR.
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Gene-Quantification.info - The reference in qPCR - Academic & Industrial Information Platform | gene-quantification.net Reviews
https://gene-quantification.net
Academic & industrial information platform for qPCR - The page describes and summarises all technical aspects involved in quantitative gene expression analysis using real-time qPCR & qRT-PCR.
gene-quantification.net
Gene Quantification & optimisation real-time / kinetic PCR/ RT-PCR reactions
http://www.gene-quantification.net/optimization.html
Real-time PCR optimisation (1). Real-time PCR optimisation (2). Real-time PCR optimisation (3). Real-time PCR optimisation (4). Real-time PCR optimisation (5). Eleven golden rules of quantitative RT-PCR. The MIQE press review. The reproducibility of biomedical research: Sleepers awake! Stephen A. Bustin,. Biomolecular Detection and Quantification, Volume 2, December 2014, Pages 35–42. Current problems in quantitative real-time RT-PCR. T Nolan, RE. Hands and SA Bustin;. Total or mRNA,. Is exacerbated by s...
Normalisation of real-time RT-PCR reactions
http://www.gene-quantification.net/hkg.html
Reference Genes / Housekeeping Genes (1). In real-time PCR - page 2. In real-time PCR - page 3. In real-time PCR - page 4. Data normalisation in real-time RT-PCR is a further major step in gene quantification analysis ( Bustin 2002. Any variation in the normalizer will obscure real changes and produce artifactual changes. And cDNA sample loading variation ( Stahlberg 2003. Pfaffl and Hageleit 2001). Or Ribogreen RNA Quantification Kit. Alternatively the rRNA content has been proposed as an optimal an...
Array and real-time PCR - BioInformatics
http://www.gene-quantification.net/array.html
Using real-time PCR for cDNA Array verification. Accuracy and calibration of commercial oligonucleotide and custom cDNA microarrays. Yuen T, Wurmbach E, Pfeffer RL, Ebersole BJ, Sealfon SC. Department of Neurology, Mount Sinai School of Medicine, New York, NY 10029, USA. Nucleic Acids Res. 2002 30(10): e48. Wurmbach E, Yuen T, Sealfon SC. Methods. 2003 31(4): 306-316. Department of Neurology, Mount Sinai School of Medicine, Box 1137, 1 Gustave L. Levy Place, New York, NY 10029, USA. Memorec Biotech GmbH,...
Gene Quantification & mRNA analysis methods & mRNAquantification
http://www.gene-quantification.net/mrna.html
Quantification of Gene Expression. Encyclopedia of DNA Element - Gene Expression Atlas - Brain Atlas - Human Proteome Maps . and more . The inclusion of transcriptome anaylsis dataset to the Human Protein Atlas database makes it even more comprehensive. Transcriptomics resources of human tissues and organs. MTD - The Mammalian Transcriptomic Database. Mass-spectrometry-based draft of the human proteome. A draft map of the human proteome. Tissue-based map of the human proteome. Looking closely at healthy ...
Gene Quantification & download REST - relative expressionsoftware tool
http://www.gene-quantification.net/download.html
QPCR Software Download page. REST - Relative Expression Software Tool:. Password protected ZIP files =. Change your computer configurations for REST implemented makros. REST basis paper in NAR 2002. Description of REST basis version. Further qPCR software applications:. GenEx - qPCR data analysis software by MultiD. A survey of tools for the analysis of qPCR data. QBase management and automated analysis of real time qPCR data. QBASE management and automated analysis of real time qPCR data. Cq calculation...
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Gene-Quantification.info - The reference in qPCR - Academic & Industrial Information Platform
BioScience Events - Dr. Michael W. Pfaffl editor@gene.quantification.info. Main focus of the GENE QUANTIFICATION web page. Is, to describe and summarize all technical aspects. Involved in quantitative gene expression analysis using real-time (kinetic) PCR & RT-PCR. Eg detection chemistry, assay specificity, sensitivity, reproducibility, irobustness,. Intra- and inter-assay variations, kinetic PCR efficiency calculation, quantification strategies,. Main focus of the PHYSIOLOGY web page. Description of qua...
Gene-Quantification.info - The reference in qPCR - Academic & Industrial Information Platform
BioScience Events - Dr. Michael W. Pfaffl editor@gene.quantification.info. Main focus of the GENE QUANTIFICATION web page. Is, to describe and summarize all technical aspects. Involved in quantitative gene expression analysis using real-time (kinetic) PCR & RT-PCR. Eg detection chemistry, assay specificity, sensitivity, reproducibility, irobustness,. Intra- and inter-assay variations, kinetic PCR efficiency calculation, quantification strategies,. Main focus of the PHYSIOLOGY web page. Description of qua...
Gene-Quantification.info - The reference in qPCR - Academic & Industrial Information Platform
BioScience Events - Dr. Michael W. Pfaffl editor@gene.quantification.info. Main focus of the GENE QUANTIFICATION web page. Is, to describe and summarize all technical aspects. Involved in quantitative gene expression analysis using real-time (kinetic) PCR & RT-PCR. Eg detection chemistry, assay specificity, sensitivity, reproducibility, irobustness,. Intra- and inter-assay variations, kinetic PCR efficiency calculation, quantification strategies,. Main focus of the PHYSIOLOGY web page. Description of qua...
Gene-Quantification.info - The reference in qPCR - Academic & Industrial Information Platform
BioScience Events - Dr. Michael W. Pfaffl editor@gene.quantification.info. Main focus of the GENE QUANTIFICATION web page. Is, to describe and summarize all technical aspects. Involved in quantitative gene expression analysis using real-time (kinetic) PCR & RT-PCR. Eg detection chemistry, assay specificity, sensitivity, reproducibility, irobustness,. Intra- and inter-assay variations, kinetic PCR efficiency calculation, quantification strategies,. Main focus of the PHYSIOLOGY web page. Description of qua...
Gene-Quantification.info - The reference in qPCR - Academic & Industrial Information Platform
Gene-Quantification.info - The reference in qPCR - Academic and Industrial Information Platform - The page describes and summarises all technical aspects involved in quantitative gene expression analysis using real-time qPCR and qRT-PCR. It presents a lot of cyclers, kits, dyes, chemistries, and services involved. Companies and institutions can present their qPCR technologies, knowledge and services right here. - gene-quantification.info.
Gene-Quantification.info - The reference in qPCR - Academic & Industrial Information Platform
BioScience Events - Dr. Michael W. Pfaffl editor@gene.quantification.info. Main focus of the GENE QUANTIFICATION web page. Is, to describe and summarize all technical aspects. Involved in quantitative gene expression analysis using real-time (kinetic) PCR & RT-PCR. Eg detection chemistry, assay specificity, sensitivity, reproducibility, irobustness,. Intra- and inter-assay variations, kinetic PCR efficiency calculation, quantification strategies,. Main focus of the PHYSIOLOGY web page. Description of qua...
Gene-Quantification.info - The reference in qPCR - Academic & Industrial Information Platform
BioScience Events - Dr. Michael W. Pfaffl editor@gene.quantification.info. Main focus of the GENE QUANTIFICATION web page. Is, to describe and summarize all technical aspects. Involved in quantitative gene expression analysis using real-time (kinetic) PCR & RT-PCR. Eg detection chemistry, assay specificity, sensitivity, reproducibility, irobustness,. Intra- and inter-assay variations, kinetic PCR efficiency calculation, quantification strategies,. Main focus of the PHYSIOLOGY web page. Description of qua...
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