Academic & industrial information platform for qPCR - The page describes and summarises all technical aspects involved in quantitative gene expression analysis using real-time qPCR & qRT-PCR.
http://www.gene-quantification.org/
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Gene-Quantification.info - The reference in qPCR - Academic & Industrial Information Platform | gene-quantification.org Reviews
https://gene-quantification.org
Academic & industrial information platform for qPCR - The page describes and summarises all technical aspects involved in quantitative gene expression analysis using real-time qPCR & qRT-PCR.
gene-quantification.org
Normalisation of real-time RT-PCR reactions
http://www.gene-quantification.org/hkg.html
Reference Genes / Housekeeping Genes (1). In real-time PCR - page 2. In real-time PCR - page 3. In real-time PCR - page 4. Data normalisation in real-time RT-PCR is a further major step in gene quantification analysis ( Bustin 2002. Any variation in the normalizer will obscure real changes and produce artifactual changes. And cDNA sample loading variation ( Stahlberg 2003. Pfaffl and Hageleit 2001). Or Ribogreen RNA Quantification Kit. Alternatively the rRNA content has been proposed as an optimal an...
Gene Quantification & mRNA analysis methods & mRNAquantification
http://www.gene-quantification.org/mrna.html
Quantification of Gene Expression. Encyclopedia of DNA Element - Gene Expression Atlas - Brain Atlas - Human Proteome Maps . and more . The inclusion of transcriptome anaylsis dataset to the Human Protein Atlas database makes it even more comprehensive. Transcriptomics resources of human tissues and organs. MTD - The Mammalian Transcriptomic Database. Mass-spectrometry-based draft of the human proteome. A draft map of the human proteome. Tissue-based map of the human proteome. Looking closely at healthy ...
Gene Quantification & download REST - relative expressionsoftware tool
http://www.gene-quantification.org/download.html
QPCR Software Download page. REST - Relative Expression Software Tool:. Password protected ZIP files =. Change your computer configurations for REST implemented makros. REST basis paper in NAR 2002. Description of REST basis version. Further qPCR software applications:. GenEx - qPCR data analysis software by MultiD. A survey of tools for the analysis of qPCR data. QBase management and automated analysis of real time qPCR data. QBASE management and automated analysis of real time qPCR data. Cq calculation...
Gene Quantification & block RT-PCR
http://www.gene-quantification.org/block.html
Polymerase Chain Reaction (PCR). Reverse Transcription - Polymerase Chain Reaction (RT-PCR). The Nobel Prize in Chemistry 1993. Polymerase Chain Reaction -. The PCR method – a copying machine for DNA molecules. Http:/ nobelprize.org/nobel prizes/chemistry/laureates/1993/illpres/pcr.html. Roche Applied Science - PCR Application Manual 3rd Edition. PCR and RT-PCR are techniques so common in today's laboratories that it is easy to forget its. Any given cycle that falls within the reaction's exponential range.
Relative expression software tool (REST)
http://www.gene-quantification.org/rest.html
Relative Expression Software Tool = REST. Today the " relative. Is increasingly used in gene expression studies, where the expression of a target gene is standardised. By a non-regulated reference-gene or by an index, containing more reference-genes. Several mathematical algorithm have been d. Eveloped to compute the expression ratio, based on real-time PCR efficiency and the. Crossing point (Ct or CP) deviation (= delta CP) of an unknown sample versus a control. But all published equations. Y Vainshtein...
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Gene-Quantification.info - The reference in qPCR - Academic & Industrial Information Platform
BioScience Events - Dr. Michael W. Pfaffl editor@gene.quantification.info. Main focus of the GENE QUANTIFICATION web page. Is, to describe and summarize all technical aspects. Involved in quantitative gene expression analysis using real-time (kinetic) PCR & RT-PCR. Eg detection chemistry, assay specificity, sensitivity, reproducibility, irobustness,. Intra- and inter-assay variations, kinetic PCR efficiency calculation, quantification strategies,. Main focus of the PHYSIOLOGY web page. Description of qua...
Gene-Quantification.info - The reference in qPCR - Academic & Industrial Information Platform
BioScience Events - Dr. Michael W. Pfaffl editor@gene.quantification.info. Main focus of the GENE QUANTIFICATION web page. Is, to describe and summarize all technical aspects. Involved in quantitative gene expression analysis using real-time (kinetic) PCR & RT-PCR. Eg detection chemistry, assay specificity, sensitivity, reproducibility, irobustness,. Intra- and inter-assay variations, kinetic PCR efficiency calculation, quantification strategies,. Main focus of the PHYSIOLOGY web page. Description of qua...
Gene-Quantification.info - The reference in qPCR - Academic & Industrial Information Platform
BioScience Events - Dr. Michael W. Pfaffl editor@gene.quantification.info. Main focus of the GENE QUANTIFICATION web page. Is, to describe and summarize all technical aspects. Involved in quantitative gene expression analysis using real-time (kinetic) PCR & RT-PCR. Eg detection chemistry, assay specificity, sensitivity, reproducibility, irobustness,. Intra- and inter-assay variations, kinetic PCR efficiency calculation, quantification strategies,. Main focus of the PHYSIOLOGY web page. Description of qua...
Gene-Quantification.info - The reference in qPCR - Academic & Industrial Information Platform
Gene-Quantification.info - The reference in qPCR - Academic and Industrial Information Platform - The page describes and summarises all technical aspects involved in quantitative gene expression analysis using real-time qPCR and qRT-PCR. It presents a lot of cyclers, kits, dyes, chemistries, and services involved. Companies and institutions can present their qPCR technologies, knowledge and services right here. - gene-quantification.info.
Gene-Quantification.info - The reference in qPCR - Academic & Industrial Information Platform
BioScience Events - Dr. Michael W. Pfaffl editor@gene.quantification.info. Main focus of the GENE QUANTIFICATION web page. Is, to describe and summarize all technical aspects. Involved in quantitative gene expression analysis using real-time (kinetic) PCR & RT-PCR. Eg detection chemistry, assay specificity, sensitivity, reproducibility, irobustness,. Intra- and inter-assay variations, kinetic PCR efficiency calculation, quantification strategies,. Main focus of the PHYSIOLOGY web page. Description of qua...
Gene-Quantification.info - The reference in qPCR - Academic & Industrial Information Platform
BioScience Events - Dr. Michael W. Pfaffl editor@gene.quantification.info. Main focus of the GENE QUANTIFICATION web page. Is, to describe and summarize all technical aspects. Involved in quantitative gene expression analysis using real-time (kinetic) PCR & RT-PCR. Eg detection chemistry, assay specificity, sensitivity, reproducibility, irobustness,. Intra- and inter-assay variations, kinetic PCR efficiency calculation, quantification strategies,. Main focus of the PHYSIOLOGY web page. Description of qua...
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